Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Filamin C (D1K3J) Rabbit mAb #86972

No. Size Price
86972S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
86972 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 290 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Filamin C (D1K3J) Rabbit mAb recognizes endogenous levels of total filamin C protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to recognize other filamin family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu1743 of human filamin C protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Filamin C (D1K3J) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).



Immunoprecipitation of Filamin C from RD cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Filamin C (D1K3J) Rabbit mAb. Western blot was performed using Filamin C (D1K3J) Rabbit mAb.


Filamins are a family of dimeric actin binding proteins that function as structural components of cell adhesion sites. They also serve as a scaffold for subcellular targeting of signaling molecules (1). The actin binding domain (α-actinin domain) located at the amino terminus is followed by as many as 24 tandem repeats of about 96 residues and the dimerization domain is located at the carboxy terminus. In addition to actin filaments, filamins associate with other structural and signaling molecules such as β-integrins, Rho/Rac/Cdc42, PKC and the insulin receptor, primarily through the carboxy-terminal dimerization domain (1-3). Filamin A, the most abundant, and filamin B are widely expressed isoforms, while filamin C is predominantly expressed in muscle (1). Filamin A is phosphorylated by PAK1 at Ser2152, which is required for PAK1-mediated actin cytoskeleton reorganization (4).

  1. Stossel, T.P. et al. (2001) Nat Rev Mol Cell Biol 2, 138-45.
  2. Tigges, U. et al. (2003) J Biol Chem 278, 23561-9.
  3. He, H.J. et al. (2003) J Biol Chem 278, 27096-104.
  4. Vadlamudi, R.K. et al. (2002) Nat Cell Biol 4, 681-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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