Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Lysine Acetyltransferase Antibody Sampler Kit #8686

REACTIVITY

No. Size Price
8686T 1 Kit ( 4 x 20 µl ) ¥3,876.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
GCN5L2 (C26A10) Rabbit mAb #3305 20 µl W,IP,IF-IC, H,M,R,Mk, B,Hr, 94 Rabbit IgG
PCAF (C14G9) Rabbit mAb #3378 20 µl W,IP,ChIP, H,M,R,Mk, B,Hr, 93 Rabbit IgG
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771 20 µl W,IP,ChIP, H,M,Mk, R, 300 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl W, Goat
CBP (D6C5) Rabbit mAb #7389 20 µl W,IP,IF-IC,ChIP, H,M,R,Mk, 300 Rabbit IgG

Specificity / Sensitivity

Each antibody in the Lysine Acetyltransferase Antibody Sampler Kit recognizes endogenous levels of respective target protein. The antibodies do not cross-react with other family members.

Lysine Acetyltransferase Antibody Sampler Kit中的每种抗体能够识别各自的内源性的目的蛋白。该抗体不会与其他家族成员产生交叉反应。

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CBP protein, human GCN5L2 protein, or human PCAF protein. Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys1535 of human CBP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

单克隆抗体经由合成的人CBP蛋白、人GCN5L2蛋白或人PCAF蛋白的氨基端氨基酸多肽免疫动物而生产的。多克隆抗体是经由合成的围绕人CBP蛋白的Lys1535残基的氨基酸多肽免疫动物而生产的。多克隆抗体经由蛋白质A和多肽亲和层析纯化而成。

Description

The Lysine Acetyltransferase Antibody Sampler Kit provides an economical means to examine several lysine acetyltrasferases, including: Acetyl-CBP, CBP, GCN5L2, and PCAF. The kit contains enough primary antibody to perform four western blots per primary.

Lysine Acetyltransferase Antibody Sampler Kit可以提供一种快速经济的方法来检测内源性赖氨酸乙酰转移酶的水平,包括Acetyl-CBP, CBP, GCN5L2, 和 PCAF。试剂盒提供的每种一抗都足够进行4次western blot实验。

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of hypo- or hyper-acetylated recombinant p300 HAT domains, either wild-type or K1499R mutant, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody (upper). Also shown in the corresponding coomassie stained SDS-PAGE gel (lower). (Details are described in Thompson, P.A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.)

Western Blotting

Western Blotting

Western blot analysis of extracts from A431, NIH/3T3, COS and PC12 cells, using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using GCN5L2 (C26A10) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30 µM) and either 20 μl of PCAF (C14G9) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells treated with Forskolin #3828 (30uM) and either 20 μl of Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using CBP (D6C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells, treated with Forskolin #3828 (30 μM, 1h) and either 20 μl of CBP (D6C5) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP® Human NR4A3 Promoter Primers #4829, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBP (D6C5) Rabbit mAb #7389.Western blot方法检测不同细胞系的提取物,所用抗体为CBP (D6C5) Rabbit mAb #7389。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771.Western blot方法检测不同细胞系的提取物,所用抗体为Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PCAF (C14G9) Rabbit mAb #3378.Western blot方法检测不同细胞系的提取物,所用抗体为PCAF (C14G9) Rabbit mAb #3378。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using GCN5L2 (C26A10) Rabbit mAb #3305.Western blot方法检测不同细胞系的提取物,所用抗体为 GCN5L2 (C26A10) Rabbit mAb #3305。

Background

CREB-binding protein (CBP) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). The role of acetylation of CBP/p300 is of particular interest (2,3). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (4). p300/CBP-associated factor (PCAF), also known as lysine acetyl-transferase 2B (KAT2B) (5), and General Control of Amino Acid Synthesis Yeast Homolog Like 2 (GCN5L2) (6) are transcriptional adaptor proteins in addition to HATs. PCAF functions as the catalytic subunit of the PCAF transcriptional co-activator complex (5). GCN5L2 functions as the catalytic subunit of the STAGA and TFTC transcription coactivator complexes (6). PCAF and GCN5L2 acetylate histone H3 at Lys14 and histone H4 at Lys8, both of which contribute to gene activation by modulating chromatin structure and recruiting additional co-activator proteins that contain acetyl-lysine binding bromo-domains (7,8). PCAF also acetylates non-histone proteins including transcriptional activators (p53, E2F1, MyoD) and general transcription factors (TFIIEβ and TFIIF) (9-12). GCN5L2 also acetylates non-histone proteins such as transcription activators (TAT, c-Myb) (13,14), transcription co-activators (PGC1-α) (15), and nuclear receptors (Steroidogenic Factor 1) (16). Acetylation of these proteins regulates their nuclear localization, protein stability, DNA binding, and co-activator association (13-16).

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications