Cell Signaling Technology

Product Pathways - Protein Stability

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb #8664

No. Size Price
8664S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
8664 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 34 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb recognizes endogenous levels of SGTA protein only when phosphorylated at Ser305.

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb兔单抗可识别内源性的Ser305磷酸化的SGTA水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser305 of human SGTA protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with λ phosphatase (+), using Phospho-SGTA (Ser305) (D23E10) Rabbit mAb (upper) and SGTA Antibody #3349 (lower).

对未处理(-)或λ磷酸酶处理(+)的HeLa细胞提取物,使用Phospho-SGTA (Ser305) (D23E10) Rabbit mAb(上图)或SGTA Antibody #3349(下图)进行Western blot分析。


SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6).


Phosphorylation of SGTA at Ser305 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (7). Site-specific mutation analysis indicated that phosphorylation at Ser305 is essential for PDGFR α stabilization and PDGFR α-dependent cancer cell survival (7).


  1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
  2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
  3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
  6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.
  7. Moritz, A. et al. (2010) Sci Signal 3, ra64.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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