Cell Signaling Technology

Product Pathways - Protein Stability

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb #8664

No. Size Price
8664S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8664 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 34 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb recognizes endogenous levels of SGTA protein only when phosphorylated at Ser305.

Phospho-SGTA (Ser305) (D23E10) Rabbit mAb兔单抗可识别内源性的Ser305磷酸化的SGTA水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser305 of human SGTA protein.

该单克隆抗体用与人类SGTA蛋白中Ser305位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with λ phosphatase (+), using Phospho-SGTA (Ser305) (D23E10) Rabbit mAb (upper) and SGTA Antibody #3349 (lower).

对未处理(-)或λ磷酸酶处理(+)的HeLa细胞提取物,使用Phospho-SGTA (Ser305) (D23E10) Rabbit mAb(上图)或SGTA Antibody #3349(下图)进行Western blot分析。

Background

SGTA, small glutamine-rich tetratricopeptide repeat-containing protein A, is an ubiquitously expressed co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity (1,2). SGTA is a 34 kDa protein that is rich in glutamine residues at its C terminus and contains three tandemly repeated TPR motifs (3). The TPR domain of SGTA shows sequence similarity to the TPR domains of Hop, CHIP, and TOM70 (4). The TPR domain of SGTA also interacts with HSP90 and was recently found to be a pro-apoptotic factor (5,6).

SGTA(小的富含谷氨酸tetratricopeptide重复序列的蛋白A),是广泛表达的分子伴侣,可以直接结合HSC70和HSP70,调节它们的ATP酶活性(1,2)。SGTA是一个34kDa的蛋白质,在羧基末端富含谷氨酸残基,包含三个串联重复TPR基序(3)。SGTA的TPR区域显示出与Hop、CHIP和TOM70中TPR区域的序列相似性(4)。SGTA的TPR区域也能和HSP90相互作用,近来也被发现是一种促凋亡因子(5,6)。

Phosphorylation of SGTA at Ser305 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (7). Site-specific mutation analysis indicated that phosphorylation at Ser305 is essential for PDGFR α stabilization and PDGFR α-dependent cancer cell survival (7).

SGTA的Ser305磷酸化位点由CST公司使用PhosphoScan®技术鉴定的,这个技术是CST的基于LC-MS/MS的磷酸化发现平台(7)。位点特异性的突变分析表明Ser305的磷酸化对于PDGFRα的稳定和PDGFRα依赖的癌细胞生存至关重要(7)。

  1. Liu, F.H. et al. (1999) J Biol Chem 274, 34425-32.
  2. Tobaben, S. et al. (2003) J Biol Chem 278, 38376-83.
  3. Cziepluch, C. et al. (1998) J Virol 72, 4149-56.
  4. Scheufler, C. et al. (2000) Cell 101, 199-210.
  5. Liou, S.T. and Wang, C. (2005) Arch Biochem Biophys 435, 253-63.
  6. Yin, H. et al. (2006) Biochem Biophys Res Commun 343, 1153-8.
  7. Moritz, A. et al. (2010) Sci Signal 3, ra64.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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