Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

IDO (D5J4E™) Rabbit mAb #86630

No. Size Price
86630S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
86630 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 43 Rabbit IgG
IP 1:200
IHC-P 1:400
F 1:800
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

IDO (D5J4E) Rabbit mAb recognizes endogenous levels of total IDO protein. Some nonspecific staining of normal breast epithelium has been observed.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IDO protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; right), using IDO (D5J4E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using IDO (D5J4E) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, overnight; right), using IDO (D5J4E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or treated with Human Interferon-γ (hIFN-γ) #8901 (10 ng/ml, 16 hr; (green) using IDO (D5J4E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using IDO (D5J4E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of IDO from extracts of HeLa cells treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is IDO (D5J4E) Rabbit mAb. Western blot analysis was performed using IDO (D5J4E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IDO (D5J4E) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IDO (D5J4E) Rabbit mAb.

Background

INDO/IDO1/indoleamine 2,3-dioxygenase (IDO) is an IFN-γ-inducible enzyme that catalyzes the rate-limiting step of tryptophan degradation (1). IDO is upregulated in many tumors and in dendritic cells in tumor-draining lymph nodes. Elevated tryptophan catabolism in these cells leads to tryptophan starvation of T cells, limiting T cell proliferation and activation (2). Therefore, IDO is considered an immunosuppresive molecule, and research studies have shown that upregulation of IDO is a mechanism of cancer immune evasion (3). The gastrointestinal stromal tumor drug, imatinib, was found to act, in part, by reducing IDO expression, resulting in increased CD8+ T cell activation and induction of apoptosis in regulatory T cells (4). In addition to its enzymatic activity, IDO was recently shown to have signaling capability through an immunoreceptor tyrosine-based inhibitory motif (ITIM) that is phosphorylated by Fyn in response to TGF-β. This leads to recruitment of SHP-1 and activation of the noncanonical NF-κB pathway (5).

  1. Yasui, H. et al. (1986) Proc Natl Acad Sci U S A 83, 6622-6.
  2. Mellor, A.L. et al. (2003) Adv Exp Med Biol 527, 27-35.
  3. Prendergast, G.C. (2008) Oncogene 27, 3889-900.
  4. Balachandran, V.P. et al. (2011) Nat Med 17, 1094-100.
  5. Pallotta, M.T. et al. (2011) Nat Immunol 12, 870-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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