Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Organelle Localization IF Antibody Sampler Kit #8653

AIF   EEA1   LAMP1   organelle   organelle marker   PDI   RCAS1  

REACTIVITY

No. Size Price
8653T 1 Kit ( 5 x 20 µl ) ¥4,653.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
RCAS1 (D2B6N) XP® Rabbit mAb #12290 20 µl W,IP,IF-IC,F, H,M,R, 32 Rabbit IgG
EEA1 (C45B10) Rabbit mAb #3288 20 µl W,IP,IF-IC, H,M,R, 170 Rabbit IgG
PDI (C81H6) Rabbit mAb #3501 20 µl W,IHC-P,IF-IC, H,M,R,Mk, 57 Rabbit
AIF (D39D2) XP® Rabbit mAb #5318 20 µl W,IP,IF-F,IF-IC, H,M,R,Mk, B,Dg, 67 Rabbit IgG
LAMP1 (D2D11) XP® Rabbit mAb #9091 20 µl W,IP,IHC-P,IF-IC, H,Mk, 42 (non-glycosylated), 90-120 (glycosylated) Rabbit IgG

Specificity / Sensitivity

Each antibody in the Organelle Localization IF Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Organelle Localization IF Antibody Sampler Kit中的每个抗体仅识别其特异的靶点,和其他家族成员没有交叉反应。每个抗体都经IF-IC验证并根据上述方法进行细胞器染色。这些蛋白的表达在不同细胞和组织中可能有所不同。请参阅www.cellsignal.com,以获得试剂盒中各个组成部分的另外的专一性/敏感性的信息。

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein, or with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, residues surrounding Ser70 of human EEA1 protein, the sequence of human PDI protein, or residues surrounding Gly147 of human RCAS1 protein.

兔单克隆抗体通过用人LAMP1蛋白的重组蛋白片段或合成肽免疫动物制备,这些合成肽是人AIF蛋白Ala520周围的残基,人EEA1蛋白Ser70周围的残基,人PDI蛋白的序列或人RCAS1蛋白Gly147周围的残基。

Description

The Organelle Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immunocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four western blots with each antibody.

Organelle Localization IF Antibody Sampler Kit通过免疫细胞化学荧光法(IF-IC)提供了一种经济的手段来鉴定细胞器。本试剂盒包含的一抗至少能进行20次IF-IC试验或每种抗体至少能进行4次western blot。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PDI (C81H6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PDI (C81H6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using PDI (C81H6) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP ™ Rabbit mAb (green), showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, C2C12, and C6 cells using AIF (D39D2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using PDI (C81H6) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using AIF (D39D2) XP® Rabbit mAb #5318 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using RCAS1 (D2B6N) XP® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; right), using RCAS1 (D2B6N) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RCAS1 (D2B6N) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human RCAS1 (hRCAS1-Myc/DDK; +), using RCAS1 (D2B6N) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of RCAS1 from HCT 116 extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or RCAS1 (D2B6N) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using RCAS1 (D2B6N) XP® Rabbit mAb.

Confocal immunofluorescent analysis of NIH/3T3 cells using PDI (C81H6) Rabbit mAb #3501 (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析NIH/3T3细胞,使用的抗体是:PDI (C81H6) Rabbit mAb #3501(绿色)、β-Actin (8H10D10) Mouse mAb #3700(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

Confocal immunofluorescent analysis of MCF7 cells, untreated (upper) or treated with Brefeldin A #9972 (5 μg/ml, 1 hr; lower), using RCAS1 (D2B6N) XP® Rabbit mAb #12290 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析MCF7细胞,未处理(上图)或用Brefeldin A #9972 (5 μg/ml, 1小时;下图)处理,使用的抗体是:RCAS1 (D2B6N) XP® Rabbit mAb #12290(绿色)。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

Confocal immunofluorescent analysis of HeLa cells using AIF (D39D2) XP® Rabbit mAb #5318 (green), showing colocalization with mitochondria that were labeled with MitoTracker® Red CMXRos #9082 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞,使用的抗体是AIF (D39D2) XP® Rabbit mAb #5318(绿色),结果显示其与MitoTracker® Red CMXRos #9082标记的线粒体处于同一位置(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb #3288 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞,使用的抗体是EEA1 (C45B10) Rabbit mAb #3288(绿色)。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb #9091 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光分析HeLa细胞,使用的抗体是LAMP1 (D2D11) XP® Rabbit mAb #9091(绿色)。肌动蛋白丝用DY-554鬼笔环肽标记(红色)。蓝色假色= DRAQ5® #4084(DNA荧光染料)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP(R) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP(R) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP(R) Rabbit mAb.

Background

Knowledge of the subcellular location of a protein may reveal its potential role in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

蛋白质的亚细胞定位知识可能揭示其潜在的在多种细胞过程中的作用。我们可以根据亚细胞位置的标记与本试剂盒中细胞器特异的抗体处于相同位置来进行亚细胞定位。这些抗体可以作为免疫荧光的有力工具,也可以用作分级分离细胞裂解物的免疫印迹对照。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MitoTracker is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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