Cell Signaling Technology

Product Pathways - Apoptosis

Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617

No. Size Price
8617S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
8617 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Mouse IgG2a
IF-F 1:50
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of lamin A and C proteins. It also reacts with the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) produced by caspase cleavage during apoptosis. This antibody does not cross-react with lamins B1 and B2.

Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗能够检测内源的lamin A 和C 蛋白。它也能检测在凋亡过程中被caspase 裂解的大片段lamin A (50 kDa)和lamin C (41 kDa) 。此抗体不与lamins B1和B2交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human lamin A protein.

该单克隆抗体是通过重组的人 lamin A 蛋白免疫动物而获得。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Lamin A/C (4C11) Mouse mAb #4777.

该 CST 抗体与Alexa Fluor® 488荧光染料偶联,并且在公司内部通过用人源细胞进行流式细胞术实验进行了证实。此抗体预计与未偶联的抗体Lamin A/C (4C11) Mouse mAb #4777具有同源交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).

流式细胞术分析 Hela 细胞,所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (绿色) 和浓度相匹配的Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (红色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

共聚焦免疫荧光分析Hela细胞,所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat brain using Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

共聚焦免疫荧光分析正常的大鼠脑组织,所用抗体为Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) 鼠单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。 Blue pseudocolor = DRAQ5® (DNA 荧光染料)。

Background

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear disregulation and cell death (5,6).

Lamin是核膜的结构组分,对维持正常的细胞功能非常重要,比如细胞周期调控、DNA修复和染色质组装(1-3)。Lamin A/C能够被caspase-6切割,可以作为caspase-6激活的标志。在凋亡过程中,Lamin A/C能够被特异地剪切成大片段(41-50 kDa)和小片段 (28 kDa)(3,4)。Lamin的剪切会引起细胞核的失调和细胞死亡(5,6)。

  1. Gruenbaum, Y. et al. (2000) J Struct Biol 129, 313-23.
  2. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  3. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  4. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  5. Oberhammer, F.A. et al. (1994) J Cell Biol 126, 827-37.
  6. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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