Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592

No. Size Price
8592S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
8592T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
8592 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 18, 43 Rabbit IgG
IP 1:100
F 1:800
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) recognizes endogenous levels of mouse caspase-8 only when cleaved at Asp387. In addition to the p18 subunit, this antibody also detects cleavage products containing the pro-domain with the p18 subunit (p43).

Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) 兔单抗能够检测内源性水平的小鼠 Asp387剪切的caspase-8蛋白。除了p18亚基外,该抗体也能识别含有p18亚基亲域(P43)的酶切产物。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp387 of mouse caspase-8 protein.

该单克隆抗体是采用合成的小鼠caspase-8蛋白天冬氨酸387位周围残基相对应的肽段免疫动物而制备的。

Western Blotting

Western Blotting

Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by hTNF-α #8902 (20 ng/ml, 4 hours), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific).

Western blot 检测CTLL-2细胞提取物,未处理 (-)或 cycloheximide (放线菌酮)(CHX, 10 μg/ml, 过夜)处理,随后用hTNF-α #8902(20 ng/ml, 4 hr)处理后,用使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (mCasp8) (+), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific).

Western blot方法检测293T细胞提取物,空质粒(-)或过表达的小鼠caspase-8 (mCasp8)(+)载体转染;使用抗体为 Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of CTLL-2 cells, untreated (red) or treated with cyclohexamide and hTNF-α #8902 (blue), using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific).

流式细胞仪检测CTLL-2细胞,未处理 (-)或hTNF-α #8902(蓝色)和 cyclohexamide (放线菌酮)处理,使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific)兔单抗。

IF-IC

IF-IC

Confocal immunofluorescent analysis of Raw 264.7 cells using Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析Raw 264.7细胞,使用抗体为Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) 兔单抗(绿色)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8,并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases,如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化,包括DNA的片段化和细胞固缩。

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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