Cell Signaling Technology

Product Pathways - Metabolism

Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb #8578

ACACB   ACC-beta   ACC2   ACCB   Acetyl-CoA carboxylase 2   acetyl-Coenzyme A carboxylase beta   Biotin carboxylase   HACC275  

No. Size Price
8578S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8578 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 280 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb recognizes endogenous levels of total acetyl-CoA carboxylase 2 protein.

Acetyl-CoA Carboxylase 2 (D5B9)兔单克隆抗体识别内源性的乙酰辅酶A羧化酶2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1416 of human acetyl-CoA carboxylase 2 protein.

该单克隆抗体用与人类乙酰辅酶A羧化酶2蛋白中Val1416位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from human adipocytes using Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb.

对人脂肪细胞抽提液使用Acetyl-CoA Carboxylase 2 (D5B9) Rabbit mAb兔单抗进行Western blot分析。

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while the 280 kDa ACC2 (ACCβ) form is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5).

乙酰辅酶A羧化酶(ACC)催化丙乙酰辅酶A羧化为丙二酰辅酶A(1)。这是脂肪酸生物合成和氧化的关键酶(1)。在啮齿类动物中,265 kDa ACC1 (ACCα)主要表达在生脂组织中,280 kDa ACC2 (ACCβ)主要表达在氧化组织中(1,2)。但是,人类中ACC2在生脂和氧化组织中都是主要的(1,2)。AMPK可以磷酸化其Ser79,PKA可以磷酸化其Ser1200,均可抑制ACC的酶活性(3)。ACC是一个抗肥胖药物的潜在靶点(4,5)。

  1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
  2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
  3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
  4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
  5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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