Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

FES (D5B4Y) Rabbit mAb #85704

No. Size Price
85704S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
85704 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 93 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

FES (D5B4Y) Rabbit mAb recognizes endogenous levels of total FES protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro422 of human FES protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using FES (D5B4Y) Rabbit mAb.

IP

IP

Immunoprecipitation of FES protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FES (D5B4Y) Rabbit mAb. Western blot analysis was performed using FES (D5B4Y) Rabbit mAb.

Background

Fes/Fps and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases (1,2). Fes and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains (3). Fes/Fps was originally identified as an oncogene from avian (Fps) and feline (Fes) retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. Mutations may activate the Fps kinase and thereby contribute to cancer (4). However, recent data strongly suggests that the c-Fes protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer (5).

  1. Smithgall, T.E. et al. (1998) Crit Rev Oncog 9, 43-62.
  2. Greer, P. (2002) Nat Rev Mol Cell Biol 3, 278-89.
  3. Sangrar, W. et al. (2005) Cancer Res 65, 3518-22.
  4. Ley, T.J. et al. (2003) Proc Natl Acad Sci U S A 100, 14275-80.
  5. Delfino, F.J. et al. (2006) J Biol Chem 281, 8829-35.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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