Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556


No. Size Price
8556S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
8556T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
8556 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 125 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine,

Specificity / Sensitivity

Phospho-FAK (Tyr397) (D20B1) Rabbit mAb detects FAK protein only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated protein such as EGFR.

磷酸化FAK (酪氨酸397) (D20B1)兔源单抗仅仅在397位酪氨酸磷酸化时检测FAK蛋白,该蛋白可能与过表达酪氨酸磷酸化蛋白如EGFR有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein.

单抗由合成的磷酸化肽段免疫动物产生,该合成肽段对应人FAK 蛋白的397位酪氨酸附近残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).未处理或经过牛小肠碱性磷酸酶(CIP)处理的A549细胞提取物使用Phospho-FAK (Tyr397) (D20B1)兔单抗(上)和FAK抗体#3285(下)进行western blot分析。


Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

黏着斑激酶(FAK)是广泛表达的细胞质蛋白酪氨酸激酶,涉及整合素调控的信号转导,它在一些生物过程包括细胞扩散、转移和存活的控制中起重要作用(1)。FAK被整合素聚集而激活,导致在397位酪氨酸的自磷酸化,这是一个Src家族激酶PI3K和PL Cγ的结合位点(2-5)。Src家族激酶的招募导致FAK催化结构域酪氨酸407, 酪氨酸576, and 酪氨酸577的磷酸化,以及FAK的C末端区域871位酪氨酸和925位酪氨酸磷酸化(6,7)。

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
  2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
  3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
  4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
  5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
  6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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