Cell Signaling Technology

Product Pathways - Protein Stability

UBQLN2 (D7R2Z) Rabbit mAb #85509

Ubiquilins   Ubiquitin Receptors  

No. Size Price
85509S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
85509 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 70 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

UBQLN2 (D7R2Z) Rabbit mAb recognizes endogenous levels of total UBQLN2 protein. This antibody does not cross-react with UBQLN1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using UBQLN2 (D7R2Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human UBQLN2 protein (hUBQLN2-Myc/DDK; +), using UBQLN2 (D7R2Z) Rabbit mAb.

IP

IP

Immunoprecipitation of UBQLN2 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is UBQLN2 (D7R2Z) Rabbit mAb. Western blot analysis was performed using UBQLN2 (D7R2Z) Rabbit mAb.

Background

Ubiquilin 2 (UBQLN2) is a broadly expressed member of the ubiquilin family of ubiquitin receptor proteins. UBQLN2 is a type 2 ubiquitin-like (UBL) protein that contains an amino-terminal UBL domain, multiple heat shock chaperonin-binding (STI) motifs, several PXX repeats, and a carboxy-terminal ubiquitin-associated (UBA) domain (1-3). Research studies indicate that the UBL domain of UBQLN2 can interact with proteasome subunits (4). The UBA domain of UBQLN2 can interact with ubiquitinated proteins and the autophagosome and allows UBQLN2 to participate in the ubiquitin-proteasome and autophagy pathways (5-8). Mutations in the PXX repeat region of the corresponding UBQLN2 gene are associated with an X-linked form of amyotrophic lateral sclerosis (ALS15) and dementia with reduced penetrance in females (9).

  1. Wu, A.L. et al. (1999) Mol Cell 4, 619-25.
  2. Kaye, F.J. et al. (2000) FEBS Lett 467, 348-55.
  3. Mah, A.L. et al. (2000) J Cell Biol 151, 847-62.
  4. Kleijnen, M.F. et al. (2000) Mol Cell 6, 409-19.
  5. Wilkinson, C.R. et al. (2001) Nat Cell Biol 3, 939-43.
  6. N'Diaye, E.N. et al. (2009) EMBO Rep 10, 173-9.
  7. N'Diaye, E.N. et al. (2009) Autophagy 5, 573-5.
  8. Lee, D.Y. et al. (2013) EMBO Rep 14, 373-81.
  9. Deng, H.X. et al. (2011) Nature 477, 211-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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