Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb #8548

No. Size Price
8548S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8548 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 320 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb detects endogenous levels of IP3 receptor only when phosphorylated at Ser1756.

只有当丝氨酸(1756位)磷酸化时,Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb兔单抗检测内源性的IP3受体。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1764 of human IP3 receptor protein. This site is equivalent to Ser1756 in the rat IP3 receptor sequence.

该单克隆抗体通过用合成肽免疫动物制备,该合成肽与人IP3 受体蛋白丝氨酸(1756位)附近的残基一样。该位点相当于大鼠IP3 受体序列中的Ser1756.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with IP3 receptor (Ser1756) phosphopeptide, or with IP3 receptor (Ser1756) non-phosphopeptide prior to incubating the membrane.

Western blot方法检测小鼠和大鼠脑组织提取物,使用的抗体为Phospho-IP3 Receptor (Ser1756) (D10E3) Rabbit mAb. 在抗体孵育之前,通过用不含肽段,或含IP3受体(Ser1756)磷酸肽或IP3(Ser1756)非磷酸化肽段进行预孵育,对该抗体的磷酸化特异性进行验证。


Inositol 1,4,5-triphosphate receptor, also known as IP3R or InsP3R, is a member of the intracellular calcium release channel family and is located in the endoplasmic reticulum. IP3R functions as a Ca2+ release channel for intracellular stores of calcium ions. There are three types of IP3 receptors (IP3R1, 2, and 3) that require the second messenger inositol 1,4,5-triphosphate (IP3) for activation (1). Four individual subunits homo- or hetero-oligomerize to form the receptor's functional channel (2). Phosphorylation of IP3R1 at Ser1756 by cyclic AMP-dependent protein kinase A (PKA) regulates the sensitivity of IP3R1 to IP3 and may be a mode of regulation for Ca2+ release (3,4). IP3R1-mediated Ca2+ release appears to have an effect on the induction of long term depression (LTD) in Purkinje cells (5).

肌醇1,4,5 - 三磷酸受体,也被称为IP3R 或InsP3R,是细胞内钙释放通道家族的成员,位于内质网中。IP3R可作为Ca2 +细胞内储存的钙离子释放通道的功能。有三种IP3受体类型(IP3R1,2,和3),第二信使激活肌醇1,4,5 - 三磷酸(IP3)(1)。四个独立的亚基同源或异源寡聚体化以形成受体的功能通道(2)。IP3R1丝氨酸(1756位)磷酸化,通过环腺苷酸AMP依赖性蛋白激酶(PKA)调节IP3R1至IP3的灵敏性,可能作为Ca2 +释放调节模型(3,4)。IP3R1介导的Ca2 +释放似乎对蒲金耶氏细胞长时程抑制(LTD)的诱导有影响(5)。

  1. Joseph, S.K. (1996) Cell Signal 8, 1-7.
  2. Galvan, D.L. et al. (1999) J Biol Chem 274, 29483-92.
  3. Haug, L.S. et al. (1999) J Biol Chem 274, 7467-73.
  4. DeSouza, N. et al. (2002) J Biol Chem 277, 39397-400.
  5. Inoue, T. et al. (1998) J Neurosci 18, 5366-73.

Application References

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