Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TBP Antibody #8515

No. Size Price
8515S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
8515 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 35-45 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TBP Antibody recognizes endogenous levels of total TBP protein.

TBP Antibody能够检测内源性TBP总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln44 of human TBP protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TBP Antibody.

使用TBP Antibody,免疫印迹(Western blot)分析不同细胞中TBP的蛋白水平。


TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex.

TATA-binding protein (TBP)是一个广泛表达的细胞核蛋白,该蛋白在一般转录因子蛋白复合物TFIID中心起作用(1-3)。TFIID包含TBP和13 TBP相关因子(TAFs),该蛋白有助于转录前起始复合物的形成,该复合物是由多种蛋白复合物 (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II)装配,在转录的起始阶段这些蛋白质结合到一个基因启动子上(1-3)。一旦前起始复合物形成,RNA polymerase II有助于延伸和基因转录。TBP通过结合到TATA-box序列从而发挥TFIID的招募功能,TATA-box序列发现在许多蛋白质编码基因的转录起始位点上游的大约25碱基对。因此,许多转录激活蛋白与TBP和多种TAF蛋白相互作用,并且有助于TFIID的招募和前起始复合物的形成。

  1. Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
  2. Berk, A.J. (2000) Cell 103, 5-8.
  3. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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