Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TBP Antibody #8515

No. Size Price
8515S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8515 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 38 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TBP Antibody recognizes endogenous levels of total TBP protein.

TBP Antibody能够检测内源性TBP总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln44 of human TBP protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TBP Antibody.

使用TBP Antibody,免疫印迹(Western blot)分析不同细胞中TBP的蛋白水平。


TATA-binding protein (TBP) is a ubiquitously expressed nuclear protein that functions at the core of the general transcription factor protein complex TFIID (1-3). TFIID, which contains TBP and 13 TBP-associated factors (TAFs), contributes to the formation of the transcription pre-initiation complex, an assembly of multiple protein complexes (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II) that bind to a gene promoter during the initiation of transcription (1-3). Once the pre-initiation complex is formed, RNA polymerase II becomes competent for elongation and transcribes the body of a gene. TBP functions in the recruitment of TFIID by binding to the TATA-box sequence found approximately 25 base pairs upstream of the transcription start site of many protein-coding genes. In addition, many transcriptional activator proteins interact with TBP and various TAF proteins to facilitate recruitment of TFIID and formation of the pre-initiation complex.

TATA-binding protein (TBP)是一个广泛表达的细胞核蛋白,该蛋白在一般转录因子蛋白复合物TFIID中心起作用(1-3)。TFIID包含TBP和13 TBP相关因子(TAFs),该蛋白有助于转录前起始复合物的形成,该复合物是由多种蛋白复合物 (TFIIA, TFIIB, TFIIE, TFIIF, TFIIH, and RNA polymerase II)装配,在转录的起始阶段这些蛋白质结合到一个基因启动子上(1-3)。一旦前起始复合物形成,RNA polymerase II有助于延伸和基因转录。TBP通过结合到TATA-box序列从而发挥TFIID的招募功能,TATA-box序列发现在许多蛋白质编码基因的转录起始位点上游的大约25碱基对。因此,许多转录激活蛋白与TBP和多种TAF蛋白相互作用,并且有助于TFIID的招募和前起始复合物的形成。

  1. Goodrich, J.A. and Tjian, R. (1994) Curr Opin Cell Biol 6, 403-9.
  2. Berk, A.J. (2000) Cell 103, 5-8.
  3. Thomas, M.C. and Chiang, C.M. (2006) Crit Rev Biochem Mol Biol 41, 105-78.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :