Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Catenin δ-1 (Ser252) Antibody #8477

No. Size Price
8477S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8477 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-Catenin δ-1 (Ser252) Antibody recognizes endogenous levels of Catenin δ-1 protein only when phosphorylated at Ser252.

磷酸化Catenin δ-1(丝氨酸252)抗体仅仅在252位丝氨酸被磷酸化时识别内源性Catenin δ-1水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser252 of human Catenin δ-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

多抗由合成的磷酸化肽段免疫动物产生,该合成肽段对应人Catenin δ-1蛋白的252位丝氨酸附近残基。抗体由蛋白A和肽段亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with λ phosphatase, using Phospho-Catenin δ-1 (Ser252) Antibody (upper) and Catenin δ-1 Antibody #4989 (lower).未处理或经过λ 磷酸酯酶处理的A-431细胞提取物,使用Phospho-Catenin δ-1 (Ser252)抗体(上)和Catenin δ-1抗体#4989(下)进行western blot分析。

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).

Catenin δ-1 (p120 catenin)有一个N氨基末端螺旋式盘旋结构域,紧跟着一个含多个磷酸化位点的调控结构域,和一个中心Armadillo重复结构域,该结构域是十个连锁的42氨基酸重复单元。C末端尾为未知功能(1)。Catenin δ-1在细胞间粘附调控履行重要作用,它在细胞表面调控E-cadherin更新,决定能对细胞粘附起作用的E-cadherin水平(2)。Catenin δ-1对cadherin调节的粘附有正向和负向效应(3)。肌动蛋白动力学机制也被catenin δ-1调控,catenin δ-1能调控RhoA, Rac, 和cdc42蛋白(1)。类似于β-catenin,catenin δ-1转位入核,虽然这种定位的作用未知,许多研究显示catenin δ-1不规则表达,或在各种类型肿瘤细胞中缺乏,暗示catenin δ-1可能作为肿瘤抑制因子其功能(4)。

Phosphorylation of Ser252 on Catenin δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (5).

Catenin δ-1在丝氨酸252位点的磷酸化由Cell Signaling Technology (CST)通过PhosphoScan®这种CST™ LC-MS/MS平台发现磷酸化位点(5)。

  1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
  2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
  3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
  4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
  5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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