Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SirT1 (1F3) Mouse mAb #8469

Deacetylase   sirt   Sirtuin  

No. Size Price
8469S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8469 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 120 Mouse IgG1
IP 1:100
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SirT1 (1F3) Mouse mAb recognizes endogenous levels of total SirT1 protein.

SirT1 (1F3) Mouse mAb鼠单抗能够检测内源性SirT1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human SirT1 protein.

通过重组人源SirT1蛋白羧基端周围相应的片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT1 (1F3) Mouse mAb.

使用SirT1 (1F3) Mouse mAb鼠单抗,免疫印迹(Western blot)分析不同细胞中SirT1 (1F3)的蛋白水平。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (left) and C2C12 (right) cells using SirT1 (1F3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

使用SirT1 (1F3) Mouse mAb 鼠单抗(绿色)标记,共聚焦免疫荧光分析HeLa (左图)和C2C12 (右图)细胞。DY-554 phalloidin标记微丝蛋白(红色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of MEF WT (left, positive) and MEF SirT1 KO (right, negative) cells usingSirT1 (1F3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School.

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryo fibroblasts (MEF) using SirT1 (1F3) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School.

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).

Silent Information Regulator (SIR2)家族基因是高度保守基因组,它们能编码烟酰胺腺呤二核苷酸依赖的蛋白去乙酰化酶,又称为组蛋白去乙酰化酶(histone deacetylase,HDAC)3。这些基因的最初发现和最好的特征是酿酒酵母( Saccharomyces cerevisiae)SIR2,它涉及交配型位点、端粒维持、DNA损伤反应和细胞老化的沉默(1)。SIRT1是哺乳动物Sir2的直系同源物,它是一个细胞核蛋白并且涉及调节许多细胞内过程,包括凋亡、细胞衰老、内分泌信号、糖稳态、老龄化和长寿 。SirT1的靶蛋白包含acetylated p53 (2,3)、p300 (4)、Ku70 (5)、forkhead (FoxO) transcription factors (5,6)、PPARγ (7)和PPARγ coactivator-1α (PGC-1α)蛋白(8)。p53和FoxO转录因子的去乙酰化作用可以抑制凋亡和增加细胞生存(2,3,5,6)。PPARγ和PGC-1α蛋白的去乙酰化作用可以调节在肝脏中糖异生/糖酵解通路和在空腹条件下白色脂肪细胞中脂肪的动态(7,8)。 SirT1去乙酰酶的活性是通过烟酰胺抑制和通过白藜芦醇激活。此外,SirT1蛋白活性可能是通过磷酸化作用调节,因为在体外Ser27和Ser47位点被磷酸化,然而,这些磷酸化位点的功能还没有被确定(9)。

  1. Guarente, L. (1999) Nat. Genet. 23, 281-285.
  2. Vaziri, H. et al. (2001) Cell 107, 149-159.
  3. Luo, J. et al. (2001) Cell 107, 137-148.
  4. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
  5. Brunet, A. et al. (2004) Science 303, 2011-2015.
  6. Motta, M.C. et al. (2004) Cell 116, 551-563.
  7. Picard, F. et al. (2004) Nature 429, 771-776.
  8. Rodgers, J.T. et al. (2005) Nature 434, 113-118.
  9. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.
  10. Joseph, A.M. et al. (2013) Exp Gerontol 48, 858-68.
  11. Li, X. et al. (2014) Sci Rep 4, 6434.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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