Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467

No. Size Price
8467S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8467 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Dog, Endogenous 78 Rabbit IgG
IP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb recognizes endogenous levels of zyxin protein only when phosphorylated at Ser142 and Ser143.

磷酸化 Zyxin (丝氨酸142/143) (D1E8)兔源单抗仅仅在丝氨酸142和丝氨酸143 磷酸化时识别内源性Zyxin 蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser142 and Ser143 of human zyxin protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 and U-2 OS cells, untreated (-) or treated (+) with λ and calf intestinal phosphatase (λ/CIP), using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb (upper) or Zyxin Antibody #3553 (lower).未处理(-)或经过λ和牛小肠碱性磷酸酶(λ/CIP)联合处理的A-431细胞提取物,使用Phospho-Zyxin (Ser142/143) (D1E8)兔单抗(上)或Zyxin抗体#3553(下)进行western blot分析。



Immunoprecipitation of extracts from A-431 cells using Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb. Western blot was performed using the same antibody. Lane 1 is 10% input.使用Phospho-Zyxin (Ser142/143) (D1E8)兔单抗对A-431细胞提取物进行免疫共沉淀。western blot使用同样的抗体。第1道使用10% input。


The zyxin family of proteins includes LIMD1, ajuba, trip6, and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2) and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5).

Zyxin家族蛋白包括LIMD1, ajuba, trip6和zyxin,每个都在C末端含三个LIM结构域。Zyxin家族成员与肌动蛋白细胞骨架有关,是细胞连接粘附复合物和整合素调控粘附复合物的元件。它们在细胞核内外穿梭,细胞核内它们可能在转录激活中起功能(1)。Zyxin涉及在焦点粘连处机械力诱导的肌动蛋白聚合的调控(2)。在粘附、迁移调控中, 很可能通过招募Ena/VASP蛋白到焦点粘附中(3)。Zyxin与肿瘤抑制因子myopodin相互作用或调控它的功能,myopodin抑制肿瘤生长和转移(4)Zyxin可能被Akt磷酸化142位丝氨酸(5)。

  1. Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
  2. Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
  3. Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
  4. Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.

Application References

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