Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human His64­1BB Ligand/TNFSF9 (hHis64-1BBL) #8460


No. Size Price
8460LC 50 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
8460LF 50 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
8460SC 10 µg ( With Carrier ) ¥1,302.00 现货查询 购买询价
8460SF 10 µg ( Carrier Free ) ¥1,302.00 现货查询 购买询价
8460 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802) was expressed in human 293 cells at Cell Signaling Technology.

CST公司利用重组的人His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802)在人的293细胞中表达。

Molecular Characterization

Recombinant N-terminally His6-tagged h4-1BBL has a calculated MW of 22,020. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides. The expected amino terminus of recombinant hHis64-1BBL was verified by amino acid sequencing.



>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis64-1BBL. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hHis64-1BBL通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 6-18 ng/ml.

重组蛋白hHis64-1BBL的生物活性是通过其在功能性ELISA实验中对4-1BB的结合能力来确定的。每个批次的ED50在6-18 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hHis64-1BBL was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hHis64-1BBL and staining overnight with Coomassie Blue.重组蛋白 hHis64-1BBL的纯度用6 µg 降解(+)和未降解(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。



The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA.重组蛋白hHis64-1BBL的生物活性是通过其在功能性ELISA实验中对4-1BB的结合能力来确定的。


Less than 0.01 ng endotoxin/1 μg hHis64-1BBL.

内毒素含量:<0.01 ng /1 μg hHis64-1BBL。


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis64-1BBL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hHis64-1BBL蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


4-1BBL (also known as TNFSF9) is a member of the TNF-R superfamily, which includes OX40L, CD27L, CD40L, and GITRL (1). 4-1BBL is expressed primarily on antigen presenting cells, such as macrophages, dendritic cells, and B cells (2). 4-1BBL binds to 4-1BB on the surface of activated T cells (1). 4-1BBL/4-1BB binding activates the NF-κB pathway via TRAF1, 2, and 3 (1). 4-1BBL functions as a co-stimulatory molecule, inducing T cell proliferation, cytolytic activity, and cytokine production (2). Matrix metalloproteases can generate soluble 4-1BBL by cleaving it from the cell surface (3). The in vitro activity of soluble 4-1BBL is enhanced by cross-linking (4,5).

4-1BBL(也叫做TNFSF9)是TNF-R超家族中的一员,此家族包括OX40L, CD27L, CD40L和GITRL(1)。4-1BBL主要在具有抗原的细胞中表达,比如巨噬细胞、树突状细胞和B细胞(2)。4-1BBL结合到4-1BB的表面并激活T细胞(1)。4-1BBL/4-1BB结合后通过TRAF1, 2和 3而激活 NF-κB信号通路(1)。4-1BBL作为供刺激因子分子,诱导T下表增殖、细胞溶解活性和细胞因子的产生(2)。基质金属蛋白能通过将其从细胞表面的剪切而引起4-1BBL的可溶性(3)。可溶性4-1BBL在体外的活性能通过结合而增强(4,5)。

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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