Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

IFN-γ (D3H2) XP® Rabbit mAb #8455

No. Size Price
8455S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
8455 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 17, 19, 23 Rabbit IgG
IP 1:50
F 1:400
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

IFN-γ (D3H2) XP® Rabbit mAb recognizes endogenous levels of total IFN-γ protein.

IFN-γ (D3H2) XP® Rabbit mAb兔单抗检测内源性IFN-γ总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human IFN-γ protein.

此单克隆抗体通过重组人源IFN-γ蛋白免疫动物制备。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood cells, untreated (left) or treated (right) with TPA #4174 (40 nM, 4 hr), Ionomycin #9995 (2 μM, 4 hr), and Brefeldin A #9972 (1 μg/mL, last 3 hr of stimulation), using a CD3 antibody and IFN-γ (D3H2) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. Analysis was performed on cells in the lymphocyte gate.对人源外周血细胞进行流式细胞术分析,未处理(左图)或处理TPA #4174 (40 nM, 4 小时), Ionomycin #9995 (2 μM, 4小时), 和 Brefeldin A #9972 (1 μg/mL,最后刺激3小时),使用抗体是CD3 antibody和FN-γ (D3H2) XP® Rabbit mAb。Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414用来作为二抗。分析基于淋巴细胞门上细胞。

IF-IC

IF-IC

Confocal immunofluorescent analysis of NK-92 cells, treated with TPA #4174 and Ionomycin #9995 (80 nM and 3 μM, 4 hr; left) or untreated (right), using IFN-γ (D3H2) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对NK-92细胞进行共聚焦分析,TPA #4174 和 Ionomycin #9995 (80 nM and 3 μM, 4小时;左图) 或未处理 (右图),使用抗体是IFN-γ (D3H2) XP® Rabbit mAb(绿色)。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with TPA #4174 (80 nM, 5 hr), Ionomycin #9995 (3 μM, 5 hr), and Brefeldin A #9972 (300 ng/mL, last 4 hr of stimulation), using IFN-γ (D3H2) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot分析NK-92细胞的细胞提取物,未处理(-)或TPA #4174 (80 nM, 5 小时), Ionomycin #9995 (3 μM, 5小时), 和 Brefeldin A #9972 (300 ng/mL, 最后4小时刺激)处理(+)。使用抗体是IFN-γ (D3H2) XP® Rabbit mAb (上图) 或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。

IP

IP

Immunoprecipitation of IFN-γ from NK-92 cell extracts, treated with TPA #4174 (80 nM, 5 hr), Ionomycin #9995 (3 μM, 5 hr), and Brefeldin A #9972 (300 ng/mL, last 4 hr of stimulation), using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IFN-γ (D3H2) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IFN-γ (D3H2) XP® Rabbit mAb.对NK-92细胞提取物中的IFN-γ进行免疫共沉淀,TPA #4174 (80 nM, 5 小时), Ionomycin #9995 (3 μM, 5小时), 和 Brefeldin A #9972 (300 ng/mL, 最后4小时刺激)处理,使用抗体是Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) or IFN-γ (D3H2) XP® Rabbit mAb (泳道3).泳道1为10%的加入量。Western blot分析使用抗体是IFN-γ (D3H2) XP® Rabbit mAb。

Background

IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4).

IFN-γ在先天免疫和获得性免疫应答中发挥重要作用。IFN-γ激活先天免疫细胞如巨噬细胞和NK细胞的细胞毒活性(1,2)。通过NK细胞和具有抗原细胞(APCs)产生IFN-γ促进了细胞介导的获得性免疫,包括诱导T淋巴细胞产生IFN-γ、增加I型和II型 MHC 分子的表达、增强肽抗原呈递(1)。IFN-γ的抗病毒活性与PKR蛋白的诱导和其它的调控蛋白有关。IFN-γ结合到 IFNGR1/IFNGR2 复合体促进了受体的二聚化并形成(IFNGR1/IFNGR2)2 -IFN-γ二聚体。这种结合诱导了受体胞内域构象的改变并激活包括Jak1、 Jak2和 Stat1 的信号通路(3)。IFN-γ的重要作用是放大免疫监控,在IFN-γ或IFNGR敲除老鼠和IFNGR1或IFNGR2失活突变体中,病原菌感染的易感性增加。IFN-γ在动脉硬化中也发挥了重要的作用(4)。

  1. Schroder, K. et al. (2004) J Leukoc Biol 75, 163-89.
  2. Martinez, F.O. et al. (2009) Annu Rev Immunol 27, 451-83.
  3. Kotenko, S.V. et al. (1995) J Biol Chem 270, 20915-21.
  4. McLaren, J.E. and Ramji, D.P. (2009) Cytokine Growth Factor Rev 20, 125-35.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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