Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-VASP (Ser157) (D1C8O) Rabbit mAb #84519

No. Size Price
84519S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
84519 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-VASP (Ser157) Rabbit mAb recognizes endogenous levels of VASP protein only when phosphorylated at Ser157.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser157 of human VASP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 and 3T3 cells, untreated (-) or Forskolin #3828-treated (10 μM, 15 min; +), using Phospho-VASP (Ser157) (D1C8O) Rabbit mAb (upper) or VASP (9A2) Rabbit mAb #3132 (lower).



Immunoprecipitation of Phospho-VASP (Ser157) from A-431 cell extracts treated with Forskolin #3828 (10 μM, 15 min). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-VASP (Ser157) (D1C8O) Rabbit mAb. Western blot analysis was performed using Phospho-VASP (Ser157) (D1C8O) Rabbit mAb. A light chain-specific secondary antibody was used.


Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

  1. Butt, E. et al. (1994) J Biol Chem 269, 14509-17.
  2. Ball, L.J. et al. (2000) EMBO J 19, 4903-14.
  3. Machesky, L.M. (2000) Cell 101, 685-8.
  4. Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35.
  5. Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25.
  6. Oelze, M. et al. (2000) Circ Res 87, 999-1005.
  7. Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6.

Application References

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