Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb #8438


No. Size Price
8438S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8438T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
8438 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 18 Rabbit IgG
F 1:1600
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198.

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗仅能够检测内源性的Asp198磷酸化的Caspase-7蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp198 of human caspase-7 protein.

该单克隆抗体是采用合成的人源caspase-7蛋白Asp198 位周围残基相对应的肽段免疫动物而制备的。



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 3 hr; right), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析HeLa细胞,未处理(左)或 Staurosporine #9953 (1 μM, 3 hr;右)处理,使用抗体为CleavedCaspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗(绿色)。DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C2C12, and H-4-II-E cells, untreated (-) or treated (+) with Staurosporine #9953 (1 μM, 3 hr), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb (upper) and Caspase-7 Antibody #9492 (lower).

Western blot 分析 HeLa,C2C12 和 H-4-II-E 细胞提取物,未处理(-)和 Staurosporine #9953 (1 μM, 3 hr)(+)处理,使用抗体为CleavedCaspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗(上图)和 Caspase-7 Antibody #9492(下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb.

流式细胞仪检测Jurkat细胞,未处理(蓝)和etoposide处理(绿),使用抗体为Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗。


Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspases-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

Caspase-7(CMH-1、Mch3、ICE-LAP3),已被确定为是细胞凋亡的主要执行者(1-4)。 Caspase-7,和caspase-3一样,是半胱天冬酶效应子,负责裂解下游底物,如(ADP-核糖)聚合酶和PARP(1,3)。在细胞凋亡中,caspase-7被上游 caspases的蛋白水解过程激活ASP23,Asp198和Asp206位点,从而产生成熟的亚基(1,3)。类似caspase -2和-3,caspase-7优先切割识别序列DEVD后的底物(5)。

  1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
  2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
  3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
  4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
  5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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