Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Rad18 (Ser403) Antibody #8393

No. Size Price
8393S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8393 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80, 90 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Phospho-Rad18 (Ser403) Antibody recognizes endogenous levels of Rad18 protein only when phosphorylated at Ser403. In some cell types, the antibody cross-reacts with a >200 kDa protein of unknown origin.Phospho-Rad18 (Ser403) Antibody能够检测内源性丝氨酸(403位)磷酸化的Rad18蛋白。在一些细胞系中,该抗体与一个大于200kDa的不明来源蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser403 of human Rad18 protein. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体是由合成的人源的针对Rad18蛋白丝氨酸(403位)的肽段免疫动物,采用蛋白A和多肽亲和层析技术纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 or HeLa cells, untreated or UV-treated, using Phospho-Rad18 (Ser403) Antibody (upper) or a total Rad18 antibody (lower).Western blot 方法检测293或Hela细胞,分为非处理组合紫外处理组,使用的抗体为Phospho-Rad18 (Ser403) Antibody (上图) 或 Rad18总蛋白抗体(下图)。

Background

DNA damage, if not repaired, can lead to genome instability and tumorigenesis. Eukaryotic cells use multiple (sometimes overlapping) signaling pathways to respond to agents that cause various types of DNA lesions. Downstream molecules in DNA repair pathways converge on the sites of DNA damage, resulting in cell cycle arrest and repair or apoptosis (1). Rad18 is an E3 ubiquitin ligase recruited to sites of DNA damage. Along with the E2 ubiquitin ligase rad6, rad18 is responsible for monoubiquitination of DNA damage proteins including the replication clamp PCNA and the Fanconi anemia core protein FANCD2. Monoubiquitination of these proteins signals to downstream effector molecules and results in the repair of either post-replication repair lesions via the translesion synthesis (TLS) pathway or of DNA double strand breaks via homologous recombination (2-4). Phospho-proteomic studies indicate that serine 403 of rad18 may be phosphorylated by ATM/ATR in response to DNA damage-inducing agents (5,6). DNA损伤,如果不进行修复,能够导致基因组不稳定和肿瘤发生。真核细胞采用多个(有时会有重叠)信号通路应答引起各种类型DNA损伤的因素。DNA修复中的下游分子集中于DNA损伤位点,引起细胞周期捕获和修复或凋亡(1)。Rad18是一种可被招募至DNA损伤位点的E3泛素连接酶。连同E2泛素连接酶rad16,rad18负责DNA损伤蛋白(包括复制夹蛋白PCNA和Fanconi贫血核心蛋白FANCD2)的单泛素化。这些蛋白的单泛素化给下游的效应器分子发送信号并且通过跨损伤合成途径进行损伤后修复或者通过同源重组修复DNA双链断裂(2-4)。磷酸蛋白质组学研究表明rad18的第403位丝氨酸可以被ATM/ATR磷酸化以响应DNA损伤诱导物(5,6)。

  1. Helleday, T. et al. (2008) Nat Rev Cancer 8, 193-204.
  2. Huang, J. et al. (2009) Nat Cell Biol 11, 592-603.
  3. Song, I.Y. et al. (2010) J Biol Chem 285, 31525-36.
  4. Ting, L. et al. (2010) DNA Repair (Amst) 9, 1241-8.
  5. Mu, J.J. et al. (2007) J Biol Chem 282, 17330-4.
  6. Matsuoka, S. et al. (2007) Science 316, 1160-6.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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