Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

PD-L2 (D7U8C™) XP® Rabbit mAb #82723

b7-dc   pd-l2   pd1l2   pdcd1lg2   pdl2   programmed cell death ligand 2  

No. Size Price
82723S 100 µl ( 10 western blots ) ¥7,500.00 现货查询 购买询价
82723T 20 µl ( 2 western blots ) ¥2,000.00 现货查询 购买询价
82723 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45-60 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

PD-L2 (D7U8C) XP® Rabbit mAb recognizes endogenous levels of total PD-L2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg168 of human PD-L2 protein.

Western Blotting

Western Blotting

Western blot analysis of HDLM-2 and Jurkat cell extracts using PD-L2 (D7U8C) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using PD-L2 (D7U8C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human mediastinal large B-cell lymphoma using PD-L2 (D7U8C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PD-L2 (D7U8C) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using PD-L2 (D7U8C) XP® Rabbit mAb.

Background

Programmed cell death 1 ligand 2 (PD-L2, B7-DC, CD273) is a member of the B7 family of cell surface ligands that regulate T-cell activation and immune responses (1,2). PD-L2 binds the PD-1 transmembrane receptor and inhibits T-cell activation. PD-L2 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by activated dendritic cells, macrophages, and T-cells (1,3). Similar in structure to related B7 family members, PD-L2 protein contains extracellular IgV and IgC2 domains, a transmembrane domain, and a short, cytoplasmic region. Research studies demonstrate that PD-L2 is expressed in several tumor types, including lung cancer, renal cell carcinoma, melanoma, Hodgkin's lymphoma and primary mediastinal large B-cell lymphoma (4-7).

  1. Latchman, Y. et al. (2001) Nat Immunol 2, 261-8.
  2. Tseng, S.Y. et al. (2001) J Exp Med 193, 839-46.
  3. Messal, N. et al. (2011) Mol Immunol 48, 2214-9.
  4. Kim, M.Y. et al. (2015) Lung Cancer 88, 24-33.
  5. Taube, J.M. et al. (2014) Clin Cancer Res 20, 5064-74.
  6. Green, M.R. et al. (2010) Blood 116, 3268-77.
  7. Ansell, S.M. et al. (2015) N Engl J Med 372, 311-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSlide is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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