Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RASAL2 (D6K9L) Rabbit mAb #82481

No. Size Price
82481S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
82481 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 150 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

RASAL2 (D6K9L) Rabbit mAb recognizes endogenous levels of total RASAL2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro69 of human RASAL2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and MDA-MB-231 cells using RASAL2 (D6K9L) Rabbit mAb.



Immunoprecipitation of RASAL2 from MDA-MB-231 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is RASAL2 (D6K9L) Rabbit mAb. Western blot analysis was performed using

RASAL2 (D6K9L) Rabbit mAb.


Small GTPases act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. RASAL2 was initially identified as a GAP for the small GTPase, Ras, and later shown to interact with the Rho-GEF ECT2, and to regulate Rho activity in human astrocytoma cells (1).

Researchers have implicated RASAL2 as a suppressor of migration and metastasis in human cancer (2), and have shown that RASAL2 downregulation promotes epithelial-mesenchymal transition and metastasis in ovarian cancer (3) and lung cancer (4). Conversely, other research studies show that RASAL2 can be oncogenic in triple negative breast cancer through activation of Rac1 signaling (5).

  1. Weeks, A. et al. (2012) Am J Pathol 181, 662-74.
  2. McLaughlin, S.K. et al. (2013) Cancer Cell 24, 365-78.
  3. Huang, Y. et al. (2014) Oncotarget 5, 6734-45.
  4. Li, N. and Li, S. (2014) Biochem Biophys Res Commun 455, 358-62.
  5. Feng, M. et al. (2014) J Clin Invest 124, 5291-304.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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