Cell Signaling Technology

Product Pathways - Companion Products

His-Tag (D3I1O) XP® Rabbit mAb (Sepharose® Bead Conjugate) #82437

No. Size Price
82437S 400 µl ( 40 immunoprecipitations ) ¥4,264.00 现货查询 购买询价
82437 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:20 All Species Expected, Transfected Only Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Specificity / Sensitivity

His-Tag (D3I1O) XP® Rabbit mAb (Sepharose® Bead Conjugate) recognizes recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the 6xHis epitope tag.

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads.

His-Tag (D3I1O) XP® Rabbit mAb (Sepharose® Bead Conjugate) is useful for the immunoprecipitation of His-tagged proteins and peptides. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated His-Tag (D3I1O) XP® Rabbit mAb #12698.

IP

IP

Immunoprecipitation of His-tagged Akt1 (Akt1-His) from COS cell extracts. Lane 1 is His-Tag (D3I10) XP® Rabbit mAb (Sepharose® Bead Conjugate) and lane 2 is 10% input. Western blot analysis was performed using His-Tag (D3I10) XP® Rabbit mAb (HRP Conjugate) #12688.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Sepharose is a registered trademark of GE Healthcare.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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