Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Stat3 (79D7) Rabbit mAb (Biotinylated) #8232

No. Size Price
8232S 100 µl ( 10 western blots ) ¥3,986.00 现货查询 购买询价
8232 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 79, 86 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Stat3 (79D7) Rabbit mAb (Biotinylated) recognizes endogenous levels of total Stat3 protein.

Stat3 (79D7) Rabbit mAb (带生物素修饰)抗体能够检测内源性Stat3总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-Stat3 fusion protein corresponding to the carboxy-terminal sequence of mouse Stat3 protein.

此单克隆抗体通过GST-Stat3可溶性蛋白免疫动物制备,这种蛋白与鼠源Stat3蛋白的羧基末端序列相一致。

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Stat3 (79D7) Rabbit mAb #4904.

CST的这种抗体能够与最优条件下的生物素相偶联。这种生物素化的抗体与未偶联的Stat3 (79D7) Rabbit mAb #4904一样,具有同物种之间的交叉反应性。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and HeLa cells using Stat3 (79D7) Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for western detection. Western blot分析 NIH/3T3 和HeLa细胞的细胞提取物,使用抗体是 Stat3 (79D7) Rabbit mAb (Biotinylated).。 Streptavidin-HRP #3999用作Western blot检测。

Background

The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

Stat3 转录因子是细胞因子和生长因子受体信号通路上重要的信号分子(1),并且对于小鼠胎儿发育是必须的(2)。Stat3 在很多的人类肿瘤中是组成性激活的(3,4)并有潜在的支配原发性癌的可能(5) 而且也具有抗细胞凋亡的活性(3)。Stat3通过Tyr705位点的磷酸化而激活,从而导致它的二聚化,入核和与DNA结合(6,7)。转录活性似乎受到 MAPK 或 mTOR 信号通路介导的Ser727位点磷酸化的调控(8,9)。Stat3不同剪切形式的表达反应了其生物学功能,Stat3α (86 kDa) 和 Stat3β (79 kDa) 形式的表达比例依赖于细胞类型、配体方向或者细胞发育的成熟阶段(10)。值得注意的是,Stat3β蛋白在C-末端转录活性区域缺少丝氨酸磷酸化位点(8)。

  1. Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120.
  2. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4.
  3. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15.
  4. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85.
  5. Bromberg, J.F. et al. (1999) Cell 98, 295-303.
  6. Darnell, J.E. et al. (1994) Science 264, 1415-21.
  7. Ihle, J.N. (1995) Nature 377, 591-4.
  8. Wen, Z. et al. (1995) Cell 82, 241-50.
  9. Yokogami, K. et al. (2000) Curr Biol 10, 47-50.
  10. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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