Cell Signaling Technology

Product Pathways - Neuroscience

KCTD12 (D8V4J) Rabbit mAb #81935

No. Size Price
81935S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
81935 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 33 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

KCTD12 (D8V4J) Rabbit mAb recognizes endogenous levels of total KCTD12 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro177 of human KCTD12 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using KCTD12 (D8V4J) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).



Immunoprecipitation of KCTD12 from U-251 MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is KCTD12 (D8V4J) Rabbit mAb. Western blot analysis was performed using KCTD12 (D8V4J) Rabbit mAb.


Potassium channel tetramerization domain-containing protein 12 (KCTD12) belongs to the family of KCTD proteins, which also contains KCTD8, 12b, and 16. These proteins are auxiliary subunits of GABAB receptors (1). The principal subunit of the GABAB receptor is formed by two GABAB receptors, which bind to GABAB ligands, couple to G proteins to inhibit adenylate cyclase production, and gate ion channels (e.g., the GIRK channels) (2). The auxiliary subunits contribute to receptor desensitization. KCTD12 produces fast desensitization by uncoupling the βγ subunits of the G protein from their effector channels (3). Research studies indicate that KCTD12 represents a biomarker with diagnostic and prognostic potential for gastrointestinal stromal tumors (4).

  1. Schwenk, J. et al. (2010) Nature 465, 231-5.
  2. Gassmann, M. and Bettler, B. (2012) Nat Rev Neurosci 13, 380-94.
  3. Turecek, R. et al. (2014) Neuron 82, 1032-44.
  4. Hasegawa, T. et al. (2013) Hum Pathol 44, 1271-7.

Application References

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