Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

PU.1 (9G7) Rabbit mAb (PE Conjugate) #81886

sc-352  

No. Size Price
81886S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
81886 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Homology

Species predicted to react based on 100% sequence homology: Pig,

Specificity / Sensitivity

PU.1 (9G7) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total PU.1 protein. This antibody does not cross react with other Ets family proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PU.1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated PU.1 (9G7) Rabbit mAb #2258.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells (blue) and THP-1 cells (green) using PU.1 (9G7) Rabbit mAb (PE Conjugate).

Background

PU.1 is a member of the Ets family of transcription factors and activates target genes through the purine-rich PU-box (1). PU.1 plays a pivotal role in the differentiation of myeloid cells and lymphocytes and is expressed in several hematopoietic cells including B lymphocytes, macrophages, neutrophils, mast cells, early erythroid cells, and megakaryocytes (1,2). The concentration of PU.1 is critical for both the determination of hematopoietic cell lineage and the regulation of differentiation versus stem cell proliferation (3,4). In addition, PU.1 activity is influenced by phosphorylation and interactions with other hematopoietic transcription factors. Phosphorylation of PU.1 at Ser146 by CK2 promotes binding to IRF4 and synergistic activation through the immunoglobulin κ 3' enhancer (5). Treatment of pro-B cells with IL-3 leads to phosphorylation of PU.1 at Ser140, resulting in increased PU.1 activity and activation of the anti-apoptotic gene MCL-1 (6). GATA1 binding blocks PU.1 activity during erythroid cell development (7). Overexpression of PU.1 resulting from proviral insertion during Friend virus infection can induce erythroleukemia, while reduced expression has been associated with acute myeloid leukemia (8).

  1. Lloberas, J. et al. (1999) Immunol Today 20, 184-9.
  2. Klemsz, M.J. et al. (1990) Cell 61, 113-24.
  3. Dahl, R. and Simon, M.C. (2003) Blood Cells Mol Dis 31, 229-33.
  4. DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-41.
  5. Pongubala, J.M. et al. (1993) Science 259, 1622-5.
  6. Wang, J.M. et al. (2003) Mol Cell Biol 23, 1896-909.
  7. Zhang, P. et al. (1999) Proc Natl Acad Sci U S A 96, 8705-10.
  8. Moreau-Gachelin, F. et al. (1988) Nature 331, 277-80.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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