Cell Signaling Technology

Product Pathways - Neuroscience

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) #8181

No. Size Price
8181S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
8181 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-F 1:50 Rat, Endogenous Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) recognizes endogenous levels of rat nestin protein.

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate)能够检测内源性的大鼠巢蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

该单克隆抗体用大鼠脊髓E15免疫动物而制备的。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat brain.

这款CST抗体标记了Alexa Fluor® 555 荧光染料,通过了内部测试可以直接用于大鼠大脑的免疫荧光试验。

IF-F

IF-F

Confocal immunofluorescent analysis of P1 rat cerebellum (left) and adult rat cerebellum (right) using Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析P1大鼠小脑(左图)和成年大鼠小脑(右图),使用的抗体是Nestin (Rat-401) Mouse mAb (Alexa Fluor® 555 Conjugate)(红色)和Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (绿色)。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Background

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.

巢蛋白是中间纤维家族的蛋白,与神经纤维蛋白结构上相关(1)。在发育的大脑中高表达,它可以调节细胞结构和细胞分裂及迁移所涉及的胞内过程(1,2)。大脑成熟后,巢蛋白的表达迅速下调,被神经纤维蛋白的表达所替代(2)。因为在成熟神经元,前体神经元和神经胶质细胞都有表达,在发育中的大脑,损伤后的大脑与脊髓中也有表达,巢蛋白作为神经干/祖细胞的标记已经得到了广泛的认可(3)。巢蛋白的表达也在多种神经肿瘤中被发现,包括胶质瘤、神经纤维瘤、星形细胞瘤,因此它作为神经肿瘤干细胞的标记也受到了认可(3)。研究发现巢蛋白也在星形细胞、视网膜、心肌、胰腺和其它组织中表达中(3)。所以,目前关于巢蛋白是神经干/祖细胞的唯一标志这一说法尚未得到大家一致肯定。

  1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71.
  2. Giliarov, A.V. (2007) Morfologiia 131, 85-90.
  3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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