Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

HMGA2 (D1A7) Rabbit mAb #8179

No. Size Price
8179S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8179 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 18 Rabbit IgG
IHC-P 1:400
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

HMGA2 (D1A7) Rabbit mAb recognizes endogenous levels of total HMGA2 protein.

HMGA2 (D1A7) Rabbit mAb兔单抗能够检测内源性HMGA2总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HMGA2 protein.

通过人工合成人源HMGA2蛋白氨基端相应的多肽片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HMGA2 (D1A7) Rabbit mAb.

使用HMGA2 (D1A7) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中HMGA2蛋白水平。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with hTGF-β1 #8915 (10 ng/ml) and hIL-1β #8900 (5 ng/ml, 48 hr; right), using HMGA2 (D1A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

使用HMGA2 (D1A7) Rabbit mAb兔单抗 (绿色),共聚焦免疫荧光分析A549细胞,细胞分为untreated (左图)或处理hTGF-β1 #8915 (10 ng/ml)和hIL-1β #8900 (5 ng/ml, 48 hr; 右图)。DY-554 phalloidin标记微丝蛋白(红色)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HCT 116 (left) or HBP-ALL (right), using HMGA2 (D1A7) Rabbit mAb.

使用HMGA2 (D1A7) Rabbit mAb兔单抗,免疫组化分析HCT 116 (左图)或HBP-ALL (右图)石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HMGA2 (D1A7) Rabbit mAb.

使用HMGA2 (D1A7) Rabbit mAb兔单抗,免疫组化分析人源结肠癌石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human uterine leiomyoma using HMGA2 (D1A7) Rabbit mAb.

使用HMGA2 (D1A7) Rabbit mAb兔单抗,免疫组化分析人源子宫肌瘤石蜡切片。

Background

HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (4-7). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (8-11).

HMGA2蛋白属于拥有AT-hook DNA结合域的高速泳动族蛋白high mobility group protein(HMG)家族。HMGA蛋白被认为是结构转录因子(architectural transcription factor);它们没有直接的转录激活能力,但是通过改变的DNA构象结合到DNA的AT富含区域从而代替调节基因表达和/huo直接与其它转录因子发生相互作用(1,2)。HMGA2蛋白大量地和广泛的表达,并且在胚胎发育期间起着关键作用(3)。HMGA2蛋白促进干细胞自我更新,以及它的表达减少是与干细胞衰老有关(4-7)。研究者已经证明在正常成年组织中HMGA2蛋白的表达水平是非常低,但是它的过表达或重新布置使与许多癌症形式有关的(8-11)。

  1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
  2. Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
  3. Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
  4. Nishino, J. et al. (2008) Cell 135, 227-39.
  5. Li, O. et al. (2006) Genesis 44, 523-9.
  6. Li, O. et al. (2007) FEBS Lett 581, 3533-7.
  7. Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
  8. Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
  9. Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
  10. Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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