Cell Signaling Technology

Product Pathways - Transcription Factors

Sox17 (D1T8M) Rabbit mAb #81778


No. Size Price
81778S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
81778 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 55 Rabbit IgG
IF-IC 1:3200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Sox17 (D1T8M) Rabbit mAb recognizes endogenous levels of total Sox17 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro272 of human Sox17 protein.



Confocal immunofluorescent analysis of SK-OV-3 (left) and HeLa (right) cells using Sox17 (D1T8M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Sox17 (D1T8M) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Expression in HeLa cells is very low, as expected.


SRY-related high mobility group box (SOX) proteins comprise a large family of widely conserved transcription factors that play important roles in development. SOX proteins possess a high mobility group (HMG) motif that binds the DNA minor groove. SOX proteins do not directly mediate transcription, but require binding partners that regulate their ability to mediate transcription of target genes that control cell fate determination and development (reviewed in 1).

Transcription factor Sox17 is a Sox family protein with an established role in endoderm specification during development (2). In addition, Sox17 plays essential roles in the maintenance of the hematopoietic stem cell pool (3) and for vascular morphogenesis during development (4,5). Sox17 negatively regulates oligodendrocyte precursor differentiation by antagonizing β-catenin signaling (6). Mutation in the corresponding Sox17 gene in humans is associated with a form of vesicoureteral reflux, a disorder characterized by congenital kidney and urinary tract defects (7).

  1. Kamachi, Y. and Kondoh, H. (2013) Development 140, 4129-44.
  2. Engert, S. et al. (2013) Development 140, 3128-38.
  3. Clarke, R.L. et al. (2013) Nat Cell Biol 15, 502-10.
  4. Lange, A.W. et al. (2014) Dev Biol 387, 109-20.
  5. Choi, E. et al. (2012) Stem Cells 30, 2297-308.
  6. Chew, L.J. et al. (2011) J Neurosci 31, 13921-35.
  7. Gimelli, S. et al. (2010) Hum Mutat 31, 1352-9.

Application References

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