Cell Signaling Technology

Product Pathways - Metabolism

Phospho-LDHA (Tyr10) Antibody #8176

Cell proliferation-inducing gene 19 protein   GSD11   L-lactate dehydrogenase A chain   lactate dehydrogenase A   lactate dehydrogenase M   LDH muscle subunit   LDH-A   LDH-M   LDH1   LDHA   LDHM   PIG19   proliferation-inducing gene 19   Renal carcinoma antigen NY-REN-59  

No. Size Price
8176S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8176 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 37 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-LDHA (Tyr10) Antibody recognizes endogenous levels of LDHA protein only when phosphorylated at Tyr10.

Phospho-LDHA (Tyr10)抗体识别内源性的Tyr10磷酸化的LDHA蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr10 of human LDHA protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与人类LDHA中Tyr10位点附近的氨基酸序列对应的人工合成肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Phospho-LDHA (Tyr10) Antibody.

对多个细胞系使用Phospho-LDHA (Tyr10)抗体进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells transfected with a construct expressing DYKDDDDK-tagged wild-type (WT) full-length human LDHA (WT hLDHA-DDK) alone or in conjuction with full-length human FGFR1 (hFGFR1), using Phospho-LDHA (Tyr10) Antibody (upper) or DYKDDDDK Tag (9A3) Mouse mAb #8146 (lower). The DYKDDDDK-tagged WT hLDHA expression construct was kindly provided by Dr. Jing Chen at Emory University School of Medicine.

对单独转染DYKDDDDk-标签的野生型(WT)全长人LDHD(WT hLDHA-DDK)或联合全长人FGFR1(hFGFR1)的293T细胞使用Phospho-LDHA (Tyr10) Antibody (上图)或DYKDDDDK Tag (9A3) Mouse mAb 鼠单抗#8146(下图)进行Western blot分析。DYKDDDDK-tagged WT hLDHA表达载体由Dr. Jing Chen at Emory University School of Medicine提供。

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

乳酸脱氢酶(LDH)催化丙酮酸和NADH转化为乳酸和NAD+。当供给线粒体制造ATP的氧气太少时,这个反应将在糖酵解中循环NADH生成NAD+。LDH在肌细胞中的主要形式是A(LDHA)亚型。LDHA启动子包括HIF-1α结合域(1)。LDHA表达在低氧情况下被诱导(2)。在高强度和长时间肌肉活动中,供氧不能满足需求,乳酸在肌细胞内堆积。当供氧恢复正常时,LDH将乳酸转化为丙酮酸并在线粒体电子传递链中产生ATP。

Recent studies indicate that LDHA is phosphorylated at Tyr10 and Tyr83 by the receptor tyrosine kinase FGFR1 (3). Phosphorylation at Tyr10 up-regulates the activity of LDHA and is found in a variety of human cancer cells (3). Results further suggest that tyrosine phosphorylation regulates the NADH/NAD+ redox homeostasis and thus promotes the Warburg effect and tumor cell growth (3).

最近的研究表明LDHA被受体酪氨酸激酶FGFR在Try10和Tyr83磷酸化(3)。Tyr10的磷酸化可以上调LDHA的活性,并且在多种人类癌细胞中发现(3)。研究结果进一步推测酪氨酸磷酸化调节NADH/NAD+ redox的动态平衡,并且促进Warburg效应和肿瘤细胞生长(3)。

  1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
  2. Semenza, G.L. (2007) Biochem J 405, 1-9.
  3. Fan, J. et al. (2011) Mol Cell Biol 31, 4938-50.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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