Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb #8174

fox0   foxo  

No. Size Price
8174S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8174 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 82-97 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb recognizes endogenous levels of FoxO3a protein only when phosphorylated at Ser413. This antibody does not cross-react with FoxO1 or FoxO4 proteins.

Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb可以识别在Ser413位点磷酸化的内源性FoxO3a 蛋白。此抗体不与FoxO1 或 FoxO4 发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser413 of human FoxO3a protein.

单克隆抗体是采用合成的与人源FoxO3的Ser413位点周围序列相对应的肽段免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells transfected with FoxO3a, untreated (-) or treated with λ phosphatase (+), using Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb (upper) and FoxO3a (75D8) Rabbit mAb #2497 (lower).

Western blot分析293T细胞提取物,采用FoxO3a转染, 未处理组(-) 或 λ 磷酸酶 (+)处理组, 所用抗体为Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb 兔单抗(上) 和 FoxO3a (75D8) Rabbit mAb兔单抗 #2497 (下).

Western Blotting

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, untreated, MG132-treated, H2O2-treated or AICAR-treated, using Phospho-FoxO3 (Ser413) (D77C9) Rabbit mAb (upper) and FoxO3a (75D8) Rabbit mAb #2497 (lower).

Western blot 分析SH-SY5Y细胞提取物, 未处理组和MG132处理组,过氧化氢处理组或AICAR处理组,所用抗体为Phospho-FoxO3 (Ser413) (D77C9) Rabbit mAb兔单抗 (上) 和FoxO3a (75D8) Rabbit mAb兔单抗 #2497 (下).

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).AMPK phosphorylates FoxO3a at Ser413 amongst other sites. While this phosphorylation event does not alter the localization of FoxO3a, it leads to activation and transcriptional activity of target genes such as Gadd45a (10).

Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生(1-3)。在这个家族中,三个成员(FoxO1,FoxO4和FoxO3a)与线虫的同源基因DAF-16具有序列相似度,而DAF-16介导包括IGFR1,PI3K和Akt在内的信号通路(4-6)。活化的forkhead成员作为肿瘤抑制因子,促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调,而这个上调过程可以被PI3K负调控(7)。forkhead转录因子被Akt在Thr24,Ser256以及Ser319磷酸化后而失活,导致出核转运与转录因子活性的抑制(8),最终导致增殖上调。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SirT1)抑制(9)。AMPK在Ser413位点磷酸化FoxO3a。然而此磷酸化不能改变FoxO3a的定位,它导致靶基因如Gadd45a的激活和转录活跃(10)。

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.
  10. Greer, E.L. et al. (2007) J Biol Chem 282, 30107-30119.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications