Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Oct-1 (D7B6) Rabbit mAb #8157


No. Size Price
8157S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8157 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 90 Rabbit IgG
IP 1:100
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

Oct-1 (D7B6) Rabbit mAb recognizes endogenous levels of total Oct-1 protein.

Oct-1 (D7B6) Rabbit mAb兔单抗识别内源性的总Oct-1蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human Oct-1 protein.


Western Blotting

Western Blotting

Westerrn blot analysis of extracts from various cell lines using Oct-1 (D7B6) Rabbit mAb.

使用Oct-1 (D7B6) Rabbit mAb兔单抗对多种细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 minutes and either 10 μl of Oct-1 (D7B6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in mediating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA-binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes, exerting an important role in development (6).

Oct-1 (POU2F1)是一个广泛表达,以八聚体形式发挥作用的转录因子,它带有一个内含同源序列的POU结构域(1)。Oct-1可以通过与一些转录因子结合介导特异基因表达,包括SNAPc(2),OBF-1 (a B-cell 转录辅助激活蛋白) (3), TFIIB (4), 和TBP (TATA-box结合蛋白) (5)。其带有的POU的 DNA结合结构域使Oct-1能够结合与招募一些组织特异性的辅助因子以正向或负向地调控不同的基因,在发育过程中发挥重要作用(6)。

  1. Sturm, R.A. et al. (1988) Genes Dev 2, 1582-99.
  2. Mittal, V. et al. (1996) Mol Cell Biol 16, 1955-65.
  3. Strubin, M. et al. (1995) Cell 80, 497-506.
  4. Nakshatri, H. et al. (1995) J Biol Chem 270, 19613-23.
  5. Zwilling, S. et al. (1994) Nucleic Acids Res 22, 1655-62.
  6. Schonemann, M.D. et al. (1998) Adv Exp Med Biol 449, 39-53.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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