Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb #8151

eIF-4B   EIF4B   Eukaryotic translation initiation factor 4B   IF4B   PRO1843  

No. Size Price
8151S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8151 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb recognizes endogenous levels of eIF4B protein only when phosphorylated at Ser406.

Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb兔单抗能够检测内源性的eIF4B总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human eIF4B protein.

该单克隆抗体经由合成的围绕人eIF4B蛋白 Ser406位点的氨基酸肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or λ phosphatase-treated (+), using Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb (upper) and eIF4B Antibody #3592 (lower).

Western blot方法检测未经处理(-)或经λ磷酸酶处理(+)的Hela细胞提取物,使用的抗体为Phospho-eIF4B (Ser406) (D1C10) Rabbit mAb兔单抗 (上图) 和 eIF4B Antibody #3592 (下图)。

Background

Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7).

真核生物起始因子4B (eIF4B)被认为在蛋白质翻译起始阶段协助eIF4F复合物。在植物中,已知eIF4B蛋白和poly-(A)结合蛋白相互作用,从而增加它与poly-(A)结合活性(1)。虽然eIF4B在胰岛素刺激下发生磷酸化和蛋白质翻译增加保持很大的相关性,但是热休克和血清饥饿能够引起eIF4B在多个位点去磷酸化,这类似一些翻译抑制的动力学特征(2-5)。许多激酶包括p70 S6 激酶能够在体外使eIF4B磷酸化,同时至少一个血清诱导的eIF4B磷酸化位点对rapamycin和LY294002敏感(6)。最近研究证明Ser406位点是通过有丝分裂素调节的新型磷酸化位点(7),同时这个磷酸化位点是依赖于MEK和mTOR通路的活化(7)。因此,这个位点的磷酸化对eIF4B翻译活性起着至关重要的作用(7)。

  1. Le, H. et al. (1997) J. Biol. Chem. 272, 16247-16255.
  2. Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481.
  3. Duncan, R.F. and Hershey, J.W. (1984) J. Biol. Chem. 259, 11882-11889.
  4. Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497.
  5. Manzella, J.M. et al. (1991) J. Biol. Chem. 266, 2383-2389.
  6. Gingras, A.C. et al. (2001) Genes Dev. 15, 807-826.
  7. van Gorp, A.G. et al. (2009) Oncogene 28, 95-106.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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