Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-Thr-Pro-Arg Motif [pTPR] MultiMab™ Rabbit mAb mix #8142

Motif   TPR  

No. Size Price
8142S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8142 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Thr-Pro-Arg Motif [pTPR] Rabbit mAb detects endogenous levels of proteins only when phosphorylated at the threonine within the TPR motif. This antibody does not cross-react with phospho-serine or phospho-threonine residues in a different context.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing the phospho-TPR motif.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated (-) or treated with Calyculin A #9902 (10 ng/ml, 20 min; +), using Phospho-Thr-Pro-Arg Motif [pTPR] Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).


The MAPK and CDK families of serine/threonine protein kinases play important roles in proliferation and cell cycle control. These kinases phosphorylate threonine or serine residues that are followed by a proline residue (1-3). MAPK phosphorylates substrates with the consensus sequence PX(S/T)P and CDKs phosphorylate substrates containing the consensus sequence (S/T)PXR/K (4,5). Some signaling molecules can be regulated by phosphorylation at a specific threonine followed by an arginine or lysine at the +2 position. For example, conventional PKC isozymes phosphorylate substrates containing a serine or threonine with Arg or Lys at the -3, -2 and +2 positions (6,7).

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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