Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-PZR (Tyr241) (D6F9) Rabbit mAb #8131

No. Size Price
8131S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8131 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Bovine, Endogenous 30-50 Rabbit IgG
IP 1:50
F 1:800
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-PZR (Tyr241) (D6F9) Rabbit mAb recognizes endogenous levels of PZR protein only when phosphorylated at Tyr241.

磷酸化PZR (酪氨酸241) (D6F9)兔源单抗仅在241位酪氨酸磷酸化时识别内源性PZR 蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr241 of human PZR protein.

单抗由合成肽段免疫动物产生,该合成肽段对应人PZR蛋白的241位酪氨酸附近残基。

IF-IC

IF-IC

Confocal immunofluorescent analysis of BAE cells, untreated (left) or H2O2-treated (2 mM, 30 min; right) using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).未处理(左)或H2O2处理(2mM,30分钟;右)的BAE细胞,使用Phospho-PZR (Tyr241) (D6F9)兔单抗(绿色)进行激光共聚焦荧光分析。蓝色假色= DRAQ5® #4084 (DNA荧光染料)。

Western Blotting

Western Blotting

Western blot analysis of extracts from BAE cells, untreated or H2O2-treated (0.3 mM, 30 min) and untreated HeLa cells using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb.未处理或H2O2处理(0.3mM,30分钟)的BAE细胞和HeLa细胞提取物使用Phospho-PZR (Tyr241) (D6F9)兔单抗进行western blot分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of BAE cells, untreated (blue) or H2O2-treated (green), using Phospho-PZR (Tyr241) (D6F9) Rabbit mAb.未处理(蓝色)或H2O2处理(绿色)的BAE细胞,使用Phospho-PZR (Tyr241) (D6F9) 兔单抗进行流式细胞分析。

Background

PZR (Protein zero related) is an immunoglobulin superfamily protein that specifically binds the tyrosine phosphatase SHP-2 through its intracellular immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1,2). PZR is phosphorylated by c-Src, c-Fyn, c-Lyn, Csk, and c-Abl (3). PP1, a Src family kinase inhibitor, inhibits PZR phosphorylation (4,5). There are three alternatively spliced isoforms, designated as PZR, PZRa, and PZRb; both PZRa and PZRb lack ITIMs (6,7). PZR is the main receptor of ConA and has an important role in cell signaling via c-Src (4). PZR is expressed in many cell types and is localized to cell contacts and intracellular granules in BAECs and mesothelioma (REN) cells. PZR has been implicated as a cell adhesion protein that may be involved in SHP-2-dependent signaling at interendothelial cell contacts (3). Hypertyrosine phosphorylation of PZR was observed during embryogenesis in a mouse model of Noonan syndrome (8).

PZR(蛋白零点相关)是免疫球蛋白超家族蛋白,通过胞内免疫受体酪氨酸为基础的抑制性基序(ITIMs)特异结合酪氨酸激酶SHP-2(1,2)。PZR 被c-Src, c-Fyn, c-Lyn, Csk, 和 c-Abl磷酸化(3)。PP1,Src家族激酶抑制剂,抑制PZR磷酸化(4,5)。有三种选择性剪接异构体,命名为PZR, PZRa, 和 PZRb;PZRa 和 PZRb缺乏ITIMs(6,7)。PZR是主要的ConA受体,通过c-Src在细胞信号中起重要作用(4)。PZR在多种细胞中表达,定位在BAECs和间皮瘤细胞的细胞接触和胞内颗粒中。PZR 涉及细胞粘附蛋白,可能涉及SHP-2依赖的信号通路内皮细胞接触中(3)。PZR的高酪氨酸磷酸化在努南综合征的小鼠模型的胚胎发育中被观察到(8)。

Upon Con A treatment or H2O2 treatment, two PZR intracellular ITIM tyrosine sites-Tyr241 and Tyr263 are phosphorylated (4,8). Phosphorylation of these two sites facilitates recruitment of SHP-2 to PZR which alters the phosphatase activity of SHP-2 and affects its downstream signaling (5,8,9).

利用ConA处理或双氧水处理,两个PZR胞内ITIM酪氨酸位点241和263被磷酸化(4,8)。这两个位点的磷酸化帮组招募SHP-2到PZR,PZR改变SHP-2的磷酸化酶活性,影响它的下游信号(5,8,9)。

  1. Zhao, Z.J. and Zhao, R. (1998) J Biol Chem 273, 29367-72.
  2. Zhao, R. and Zhao, Z.J. (2000) J Biol Chem 275, 5453-9.
  3. Kusano, K. et al. Endothelium 15, 127-36.
  4. Zhao, R. et al. (2002) J Biol Chem 277, 7882-8.
  5. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.
  6. Zannettino, A.C. et al. (2003) Biochem J 370, 537-49.
  7. Zhao, R. and Zhao, Z.J. (2003) Biochem Biophys Res Commun 303, 1028-33.
  8. Eminaga, S. and Bennett, A.M. (2008) J Biol Chem 283, 15328-38.
  9. Zhao, R. et al. (2003) J Biol Chem 278, 42893-8.

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