Product Pathways - Cell Cycle / Checkpoint
Cyclin F (D9K2U) Rabbit mAb #81045
|81045S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|81045||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
Cyclin F (D9K2U) Rabbit mAb recognizes endogenous levels of total Cyclin F protein. This antibody recognizes an unidentified protein of 80 kDa by western blot.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro665 of human Cyclin F protein.
Western blot analysis of extracts from HT-29 and U-2 OS cells, untreated (-) or synchronized by double thymidine block followed by an eight hour release into complete medium (+), using Cyclin F (D9K2U) Rabbit mAb (upper) or α-Actinin (D6F6) Rabbit mab #6487 (lower).
Cyclin F is the founding member of the F-box protein family, present in all eukaryotic cells. F-box proteins are components of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. The substrate specificity of the SCF complex is determined by the interchangeable F-box proteins, which act as adaptors by associating with phosphorylated substrate proteins and recruiting them to the SCF core (1).
Cyclin F contains a cyclin box domain in addition to an F-box domain, but does not regulate the activity of cyclin dependent kinases. Cyclin F expression does oscillate during the cell cycle, however, peaking in G2 phase (2).
Cyclin F interacts with the centrosomal protein CP110, which plays critical roles centriole duplication and spindle formation. Cyclin F-mediated degradation of CP110 in G2 phase is required for normal progression into mitosis (3). In response to ionizing radiation, which causes DNA double strand breaks, Cyclin F interacts with B-Myb, preventing cyclin A-dependent phosphorylation of B-Myb, and delaying progression into mitosis. This G2 phase arrest allows the cell to respond to the DNA damage-induced G2/M phase checkpoint (4). Cyclin F also controls the stability of the ribonucleotide reductase M2 subunit, RRM2, which functions in maintaining the levels of dNTPs available in the cell for DNA synthesis and repair, in response to genotoxic stress (5). Researchers have implicated cyclin F as a prognostic marker in hepatocellular carcinoma (HCC) (6).
- Reed, S.I. (2003) Nat Rev Mol Cell Biol 4, 855-64.
- D'Angiolella, V. et al. (2013) Trends Cell Biol 23, 135-40.
- D'Angiolella, V. et al. (2010) Nature 466, 138-42.
- Klein, D.K. et al. (2015) Nat Commun 6, 5800.
- D'Angiolella, V. et al. (2012) Cell 149, 1023-34.
- Fu, J. et al. (2013) Cancer Sci 104, 508-15.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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