Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

TFIIF-α Antibody #8080

TF2   TF2F   TFII  

No. Size Price
8080S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
8080 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 74 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

TFIIF-α Antibody recognizes endogenous levels of total TFIIF-α protein.

TFIIF-α Antibody能够检测内源性TFIIF-α总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TFIIF-α protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TFIIF-α Antibody.

使用TFIIF-α Antibody,免疫印迹(Western blot)分析不同细胞系中TFIIF-α的蛋白水平。


TFIIF is a member of the group of general transcription factors that facilitate the binding of RNA polymerase II (Pol II) to promoter sequences as part of the pre-initiation complex (PIC) (1). TFIIF consists of subunits TFIIF-α (RAP74) and TFIIF-β (RAP30). It is involved in the stabilization of Pol II association with the PIC and selection of the transcription start site during transcription initiation (1,2). In addition to its role in transcription initiation, TFIIF has been shown to stimulate the transcription elongation activity of Pol II as well as dephosphorylation and recycling of Pol II during transcription termination (3-5).

TFIIF是一个通用转录因子群组的成员,它有助于RNA polymerase II (Pol II)结合到启动子序列上作为起始前复合物(PIC)的一部分(1)。TFIIF由TFIIF-α (RAP74)和TFIIF-β (RAP30)亚基组成。在转录起始阶段该蛋白涉及到Pol II联系着PIC和转录起始位点的选择的稳定作用(1,2)。除了在转录起始的作用外,TFIIF已经证明能够刺激Pol II的转录延伸活性和去磷酸化以及在转录终止时的Pol II的重新回收(3-5)。

  1. Thomas, M.C. and Chiang, C.M. Crit Rev Biochem Mol Biol 41, 105-78.
  2. Hahn, S. (2004) Nat Struct Mol Biol 11, 394-403.
  3. Sims, R.J. et al. (2004) Genes Dev 18, 2437-68.
  4. Zhang, C. and Burton, Z.F. (2004) J Mol Biol 342, 1085-99.
  5. Zawel, L. et al. (1995) Genes Dev 9, 1479-90.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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