Product Pathways - Transcription Factors
BACH2 (D3T3G) Rabbit mAb #80775
|80775S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|80775||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,
Specificity / Sensitivity
BACH2 (D3T3G) Rabbit mAb recognizes endogenous levels of total BACH2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala304 of human BACH2 protein.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 RL-7 cells and either 10 μl of BACH2 (D3T3G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human PRDM1 promoter primers, SimpleChIP® Human HMOX1 Promoter Primers #53538, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Western blot analysis of extracts from various cell lines using BACH2 (D3T3G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders including type 1 diabetes, celiac disease, Crohn’s disease, and the skin condition known as vitiligo (7-10).
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- Muto, A. et al. (1998) EMBO J 17, 5734-43.
- Muto, A. et al. (2004) Nature 429, 566-71.
- Ochiai, K. et al. (2006) J Biol Chem 281, 38226-34.
- Muto, A. et al. (2010) EMBO J 29, 4048-61.
- Shaffer, A.L. et al. (2002) Immunity 17, 51-62.
- Cooper, J.D. et al. (2008) Nat Genet 40, 1399-401.
- Dubois, P.C. et al. (2010) Nat Genet 42, 295-302.
- Franke, A. et al. (2010) Nat Genet 42, 1118-25.
- Jin, Y. et al. (2012) Nat Genet 44, 676-80.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 4486 SimpleChIP® Human α Satellite Repeat Primers
- 53538 SimpleChIP® Human HMOX1 Promoter Primers
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7017 6-Tube Magnetic Separation Rack
- 7071 Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9004 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
- 9005 SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
- 9006 ChIP-Grade Protein G Magnetic Beads
- 9007 ChIP-Grade Protein G Agarose Beads
For Research Use Only. Not For Use In Diagnostic Procedures.
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SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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