Cell Signaling Technology

Product Pathways - Transcription Factors

BACH2 (D3T3G) Rabbit mAb #80775

No. Size Price
80775S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
80775 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 130 Rabbit IgG
IP 1:200
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Specificity / Sensitivity

BACH2 (D3T3G) Rabbit mAb recognizes endogenous levels of total BACH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala304 of human BACH2 protein.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 RL-7 cells and either 10 μl of BACH2 (D3T3G) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human PRDM1 promoter primers, SimpleChIP® Human HMOX1 Promoter Primers #53538, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using BACH2 (D3T3G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of BACH2 from Daudi cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BACH2 (D3T3G) Rabbit mAb. Western blot analysis was performed using BACH2 (D3T3G) Rabbit mAb.

Background

The transcription regulator BTB and CNC homolog 2 (BACH2) is a bZIP domain-containing transcriptional repressor that dimerizes with MafK and binds Maf recognition elements (MAREs) to regulate transcription (1,2). BACH2 is part of a network of transcription factors that controls the transition of activated B cells into either antibody-producing plasma cells or memory B cells (3-5). Plasma cell differentiation requires the transcription factor Blimp1 (6). BACH2 suppresses expression of Blimp1 in activated B cells, which delays plasma cell differentiation and allows time for class switch recombination and somatic hypermutation (3-5). Genome-wide association studies have linked the genetic locus containing BACH2 to several immune-related disorders including type 1 diabetes, celiac disease, Crohn’s disease, and the skin condition known as vitiligo (7-10).

  1. Oyake, T. et al. (1996) Mol Cell Biol 16, 6083-95.
  2. Muto, A. et al. (1998) EMBO J 17, 5734-43.
  3. Muto, A. et al. (2004) Nature 429, 566-71.
  4. Ochiai, K. et al. (2006) J Biol Chem 281, 38226-34.
  5. Muto, A. et al. (2010) EMBO J 29, 4048-61.
  6. Shaffer, A.L. et al. (2002) Immunity 17, 51-62.
  7. Cooper, J.D. et al. (2008) Nat Genet 40, 1399-401.
  8. Dubois, P.C. et al. (2010) Nat Genet 42, 295-302.
  9. Franke, A. et al. (2010) Nat Genet 42, 1118-25.
  10. Jin, Y. et al. (2012) Nat Genet 44, 676-80.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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