Cell Signaling Technology

Product Pathways - Neuroscience

Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb #8068


No. Size Price
8068S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8068 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 32 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb recognizes endogenous levels of GGA3 protein only when cleaved at Asp313.

Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb 兔单抗可识别内源性的在Asp313位点切割的GGA3。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp313 of human GGA3 protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with staurosporine #9953 (1 μM, 3 hrs), using Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb (upper) and GGA3 Antibody #4167 (lower).

Western blot分析HeLa细胞(未处理或staurosporine #9953处理,1μM,3小时),使用的抗体是Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb 兔单抗(上图)或GGA3 Antibody 兔多抗#4167(下图)。


GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).

GGA3是包括GGA1和GGA2的GGA蛋白家族成员之一。这些蛋白质包括四个不同的部分:VHS结构域,结合Di-亮氨酸排序信号DXXLL; GAT结构域结合ARF-GTP; 铰链区招募clathrin; GAE结构域具有与γ-adaptin相似的序列,可以招募很多蛋白质。Arf1-GTP酶招募GGA3到高尔基体反面网络。GGAS分拣酸水解酶到溶酶体中,参与从高尔基复合体到内吞体运送含有DXXLL信号的蛋白质(1)。在细胞凋亡或脑缺血时,GGA3被caspase-3在Asp313位置裂解,减少GGA3水平和溶酶体降解β-分泌酶(BACE)的水平。BACE的活性增加和数量增多在β-淀粉样蛋白(Aβ)的形成中发挥作用,在阿尔茨海默氏病中,BACE的升高和Aβ的积累相一致(2)。

  1. Bonifacino, J.S. (2004) Nat Rev Mol Cell Biol 5, 23-32.
  2. Tesco, G. et al. (2007) Neuron 54, 721-37.

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