Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

NRBF-2 Antibody #8066

COPR   COPR-2   NRBF-2  

No. Size Price
8066S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
8066 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

NRBF-2 Antibody recognizes endogenous levels of total NRBF-2 protein. This antibody may cross-react with NRBF-1/COPR-1.NRBF-2 Antibody识别内源性水平的总NRBF-2蛋白。该抗体可能与NRBF-1/COPR-1发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu145 of human NRBF-2 protein. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体通过用合成肽免疫动物制备,该合成肽是人NRBF-2蛋白亮氨酸(145位)附近的残基。抗体由蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NRBF-2 Antibody.Western blot方法检测多个细胞系的提取物,使用的抗体为NRBF-2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged full-length human NRBF-2 construct (+), using NRBF-2 Antibody.Western blot方法检测293T细胞的提取物,细胞不转染(-)或用Myc/DDK标记的全长人NRBF-2构建体转染(+),使用的抗体为NRBF-2 Antibody.


Nuclear Receptor Binding Factor-2 (NRBF-2), also referred to as Comodulator of PPAR and RXRα-2 (COPR-2), has been shown to interact with the AF-2 region of several nuclear hormone receptors with varying affinities such as PPARα, RARα, RARγ, and RXRα (1,2). NRBF-2 contains a LLYLL motif, which matches the LXXLL NR box consensus and is required for functional NRBF-2/nuclear receptor complex formation and repression of receptor function. NRBF-2 also contains a unique autonomous activation domain and thus, does not completely abrogate nuclear receptor function, suggesting that NRBF-2 might serve as a molecular rheostat to fine-tune the transcriptional activity of liganded nuclear receptors (1,2).核受体结合因子-2(NRBF-2)也被称为PPAR和RXRα-2(COPR-2)的共调节子,已被证明其与一些核激素受体AF-2区域有不同的亲和力,如PPARα、RARα、 RARγ 和RXRα(1,2)。 NRBF-2包含一个LLYLL模体,与LXXLL NR框相匹配,对于功能性NRBF-2/核受体复合物的形成和抑制受体功能是需要的。NRBF-2还包含一个独特的自主激活结构域,因此不能完全去除核受体的功能,这表明NRBF-2可能作为一个分子可变电阻器,以微调已配位核受体的转录活性(1,2)。

  1. Yasumo, H. et al. (2000) Biochim Biophys Acta 1490, 189-97.
  2. Flores, A.M. et al. (2004) J Invest Dermatol 123, 1092-101.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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