Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Vascular Endothelial Growth Factor-165 (hVEGF165 ) #8065

vegf   VEGF-165   VEGF165  

No. Size Price
8065LC 50 µg ( With Carrier ) ¥7,768.00 现货查询 购买询价
8065LF 50 µg ( Carrier Free ) ¥7,768.00 现货查询 购买询价
8065SC 10 µg ( With Carrier ) ¥2,580.00 现货查询 购买询价
8065SF 10 µg ( Carrier Free ) ¥2,580.00 现货查询 购买询价
8065 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human VEGF165 (hVEGF165) Ala207-Arg371 (Accession #NP_001020539) was expressed in human 293 cells at Cell Signaling Technology.

CST公司在人293细胞中生产重组人蛋白VEGF165 (hVEGF165) Ala207-Arg371 (Accession #NP_001020539)。

Molecular Characterization

Recombinant hVEGF165 contains no "tags" and has a calculated MW of 19,165. DTT-reduced protein migrates as a 24 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 40 kDa protein. The expected amino-terminal APMAE of recombinant hVEGF165 was verified by amino acid sequencing.

重组的hVEGF165蛋白没有标签,未糖基化蛋白的分子量据推算为19,165Da。DTT-还原蛋白迁移时是作为24kDa 的多肽而转移。未还原的蛋白通过胱氨酸形成二聚体并作为40kDa 多肽而迁移。重组hVEGF165蛋白的N-末端APMAE的序列通过测序得到。


>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hVEGF165. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) hVEGF165通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。


The bioactivity of recombinant hVEGF165 was determined in a cell proliferation assay using HUVEC. The ED50 of each lot is between 1-6 ng/ml.

重组蛋白hVEGF165的生物活性是通过HUVEC细胞的细胞增殖实验确定的。每个批次的ED50在1-6 ng/ml之间。

Coomassie Gel

Coomassie Gel

The purity of recombinant hVEGF165 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hVEGF165 and staining overnight with Coomassie Blue.重组蛋白hVEGF165的纯度用6 µg还原(+) 和未还原(-)的蛋白走SDS-PAGE 胶来观察。考马斯亮蓝染色过夜。



The proliferation of HUVEC treated with increasing concentrations of hVEGF165 was assessed. After 72-hour treatment with hVEGF165 cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.用递增浓度的hVEGF165处理HUVEC 并验证增殖的实验。用 hVEGF165 处理72小时后,细胞与四唑盐孵育,然后测定OD450 - OD650数值。

Western Blotting

Western Blotting

Western blot analysis of extracts from HUVEC untreated or treated with hVEGF165 for 15 minutes, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 (upper) and p38 MAPK Antibody #9212 (lower).Western免疫印迹。用Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215(上图)和p38 MAPK Antibody #9212(下图)研究未经处理和经hVEGF165 处理15分钟的 HUVEC细胞的细胞提取液。


Less than 0.01 ng endotoxin/1μg hVEGF165.

内毒素含量:<0.01 ng /1 μg hVEGF165


With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hVEGF165. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hVEGF165蛋白溶解在包含 20 μg BSA的PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。

无载体:每微克hVEGF165蛋白溶解在PBS(pH 7.2)溶液中,并通过 0.22 μm 滤膜冻干。


VEGF165 is the most abundant splice variant of VEGF-A (1,2). VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability (1). VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes (1,2). VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix (2,3). VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2 (1). VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 (1-3) that function to enhance VEGFR2 signaling (1). Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).

VEGF165是VEGF-A不同剪切中数量最丰富的形式(1,2)。VEGF165是通过很多细胞产生包括内皮细胞、巨噬细胞、T细胞。VEGF165参与到了血管再生、血管内皮细胞生存、生长、迁移和血管渗透(1)。VEGF基因的表达受到组织缺氧,炎症因子和肿瘤组织产生的致癌基因的诱导(1-3)。VEGF165结合到硫酸乙酰肝素并返回细胞表面和细胞间质中(2,3)。VEGF165结合到VEGFR1和VEGFR2 受体酪氨酸激酶(1)。VEGF165是唯一能够结合到共受体NRP-1和NRP-2上(1-3)并增强VEGFR2信号(1)。 VEGF165 结合到 VEGFR1和VEGFR2导致了信号通路的激活,如PI3K/AKT、P38 MAPK和桩蛋白(1)。在很多癌症中,VEGF在肿瘤血管再生中发挥了重要的作用(2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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