Cell Signaling Technology

Product Pathways - Immunohistochemistry

SignalStain® DAB Substrate Kit #8059

chromogen   DAB   diaminobenzidine   ihc   substrate  

REACTIVITY

No. Size Price
8059P 1 Kit ( 120 slides ) ¥506.00 现货查询 购买询价
8059S 1 Kit ( 1200 slides ) ¥1,672.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
SignalStain® DAB Diluent 12 ml
SignalStain® DAB Chromogen Concentrate 360 µl

Description

The SignalStain® DAB Substrate Kit contains all of the necessary reagents to prepare a working solution of diaminobenzidine (DAB) for staining tissue sections. The DAB working solution reacts with peroxidase (HRP) detection systems such as the SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125), yielding a brown reaction product.

此试剂盒包含所有DAB染色所需要的试剂。工作液与HRP检测系统反应,比如SignalStain® Boost IHC Detection Reagents (HRP, Rabbit #8114, and HRP, Mouse #8125),产生棕色显色。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.免疫组化分析人卵巢癌石蜡切片,使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145.免疫组化分析人毛细血管瘤石蜡切片,使用的抗体是Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858.免疫组化分析人乳腺癌石蜡切片,使用的抗体是Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858。

Directions for Use

Storage: Substrate kit should be stored at 4ºC, protected from light. Each kit is stable for 12 months upon receipt if the reagents are not combined.

To prepare the DAB working solution, add 1 drop (30 μl) SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent and mix well before use. Working solutions are stable for up to 14 days when stored at 4ºC or up to 5 days when stored at room temperature (approximately 25ºC). While the solution may change color over that time period, it will have no effect on the quality of the staining.

*Please note that when using 8059P, a dropper for the chromogen concentrate is not included. Use a pipet to add 30 μl of SignalStain® DAB Chromogen Concentrate to 1 ml SignalStain® DAB Diluent.

Important: DAB is a suspected carcinogen. Good laboratory practices should be followed, and appropriate protective equipment should be used when handling DAB, including gloves, protective eyeware, and a lab coat. Dispose in accordance with local regulations.

After incubation with SignalStain® Boost IHC Detection Reagent or other HRP-based detection systems, wash slides and incubate with the working solution at room temperature until staining develops. 1-10 minutes generally provides an acceptable staining intensity.

Wash slides in water for 5 minutes.

Counterstain, if desired, and mount coverslips with either a permanent mounting media or an aqueous-based mounting media. Please note, permanent mounting requires dehydration through ethanol and cleaning through xylene. Please note, permanent mounting requires dehydration through ethanol and clearing through xylene.

储存: Substrate kit应该存储在4ºC,免受光。如果试剂不融合,每个试剂盒可稳定12个月。准备DAB工作液,加1滴(30μl) SignalStain® DAB Chromogen Concentrate到1毫升S SignalStain® DAB Diluent并混合使用。工作液可储存在4ºC14天或在室温下(大约25ºC) 储存5天。储存液在那个时期可能会改变颜色,但它对染色的质量没有影响。注意,当使用8059p, chromogen concentrate不包括滴管。用吸管加30μl SignalStain® DAB Chromogen Concentrate到1毫升SignalStain® DAB Diluent中。重要提示:DAB疑似是一个致癌物。应遵循良好的实验室规范,应该使用适当的防护设备处理,包括手套、防护眼镜,和实验服。按照当地规定处理。SignalStain® Boost IHC Detection Reagent或其他基于HRP的检测系统孵育后,洗片并在室温下与工作液孵育到染色。1 - 10分钟通常提供一个可接受的染色强度。在水中洗片5分钟。复染,如果需要,永久封片或水封片。请注意,永久封片需要通过乙醇脱水和二甲苯清洁。

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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