Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) #8058

sc-5546  

No. Size Price
8058S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
8058 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Mouse IgG1
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total α-tubulin protein.

α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)检测内源性α-tubulin总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts. α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)recognizes residues surrounding Val440.

通过纯化大脑提取物得到的全长的鸡α-tubulin蛋白去免疫动物从而制备出单克隆抗体。α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate)识别Val440位点残基。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated α-Tubulin (DM1A) Mouse mAb #3873.

Cell Signaling Technology antibody连接着Alexa Fluor® 488 fluorescent dye,并且直接用流式细胞仪分析方法内部检测人类细胞。该抗体被期望与非连接的α-Tubulin (DM1A) Mouse mAb #3873有相同的物种交叉反应。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (绿色)标记,共聚焦免疫荧光分析HeLa细胞。DY-554 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).

与Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (红色)比较,使用α-Tubulin (DM1A) Mouse mAb (Alexa Fluor® 488 Conjugate) (蓝色)标记,流式细胞仪分Jurkat细胞。

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

细胞骨架由三种类型的细胞质基质纤维组成:微管、微丝(肌动蛋白纤维)和中间纤维。球形微管蛋白亚基包含微管建筑块,由α/β-tubulin异源二聚体形成所有真核细胞的tubulin亚单位。γ-tubulin被需要在tubulin亚单位的成核聚合作用中去形成微管聚合物。许多细胞运动是通过微管动力介导的,包括纤毛和鞭毛的跳动、膜泡的核质转运、在减数分裂/有丝分裂期间染色体排布和神经轴突的迁移。这些运动由于竞争性微管聚合作用和解聚作用或通过微管动力蛋白的运动(1)。

  1. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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