Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053

Jun   JunB  

No. Size Price
8053S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8053 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 43 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb recognizes endogenous levels of JunB protein when phosphorylated at Thr102 and/or Thr104. The antibody does not detect non-phosphorylated JunB protein.

Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb兔单抗只能检测内源的在Thr102 和/或 Thr104位点磷酸化的JunB蛋白。此抗体不与非磷酸化的JunB蛋白交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr102 and Thr104 of human JunB protein.

此单克隆抗体是通过合成人源对应的JunB Thr102和Thr104位点周围的磷肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells treated with Anisomycin (25 ug/ml) for the time indicated, with or without pretreatment with JNK inhibitor SP600125 (40 min, 50 uM), using Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb (upper) or JunB (C37F9) Rabbit mAb #3753 (lower).

Background

JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).

JunB是一个碱性亮氨酸拉链(bZIP)转录因子,属于Jun家族,能够诱导c-Jun和JunD。Jun家族的成员与Fos 和ATF蛋白形成具有转录因子功能同源或异源二聚体AP-1(激活蛋白1),其活性通过各种生理和病理的刺激如生长因子,感染和胁迫信号(1-4)。JunB有时能够对抗c-Jun转录活性,在小鼠的发育中,它可能在功能上取代c-jun (5-7)。JunB调控造血干细胞数目并在慢性骨髓性白血病的发病机理(CML)和急性骨髓性白血病(AML)中起了重要的作用(8,9)。

JunB expression is selectively induced in T helper 2 (Th2) cells during T cell differentiation. JunB interacts with c-Maf, and the resulting complex functions synergistically to activate transcription of Interleukin-4 (IL-4), one of the signature cytokines secreted by Th2 cells. Transcriptional regulation of IL-4 was shown to be enhanced by JNK-mediated phosphorylation of JunB at Thr102 and Thr104 (10). Phosphorylation of these residues enhances DNA binding of the JunB/c-Maf complex at the P1 regulatory site of the IL-4 promoter, leading to Th2-restricted IL-4 expression.

在T细胞的分化中,JunB选择性的在T细胞的助细胞2(Th2)中诱导表达。JunB与c-Maf相互作用形成复合体并协同作用从而激活白介素4(IL-4)的转录,这是Th2所分泌的特征因子之一。对IL-4的转录调控通过JNK介导的在JunB Thr102和Thr104位点的磷酸化而得到加强(10)。在这些位点的磷酸化加强了DAN与JunB/c-Maf复合体在IL-4启动子的P1调控位点的结合,从而导致了Th2限制了IL-4的表达。

  1. Busch, S.J. and Sassone-Corsi, P. (1990) Trends Genet. 6, 36-40.
  2. Shaulian, E. and Karin, M. (2002) Nat. Cell Biol. 4, E131-E136.
  3. Hess, J. et al. (2004) J. Cell Sci. 117, 5965-5973.
  4. Mechta-Grigoriou, F. et al. (2001) Oncogene 20, 2378-2389.
  5. Chiu, R. et al. (1989) Cell 59, 979-986.
  6. Schütte, J. et al. (1989) Cell 59, 987-997.
  7. Passegué, E. et al. (2002) Nat. Genet. 30, 158-166.
  8. Steidl, U. et al. (2006) Nat. Genet. 38, 1269-1277.
  9. Passegué, E. et al. (2004) Cell 119, 431-443.
  10. Li, B. et al. (1999) EMBO J 18, 420-32.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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