Cell Signaling Technology

Product Pathways - Metabolism

OPA1 (D6U6N) Rabbit mAb #80471

OPA   optic atophy   sc-367890  

No. Size Price
80471S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
80471 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 80-100 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

OPA1 (D6U6N) Rabbit mAb recognizes endogenous levels of total OPA1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu821 of human OPA1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts of MEFs from wild-type (-) and OPA knockout (OPA-/-; +) mice using OPA1 (D6U6N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OPA1 (D6U6N) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® OPA1 siRNA I (+), or SignalSilence® OPA1 siRNA II using OPA1 (D6U6N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The OPA1 (D6U6N) Rabbit mAb confirms silencing of OPA1 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

IP

IP

Immunoprecipitation of OPA1 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is OPA1 (D6U6N) Rabbit mAb. Western blot analysis was performed using OPA1 (D6U6N) Rabbit mAb. A conformation specific secondary antibody was used to avoid reactivity with IgG.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and HeLa cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP; +) using OPA1 (D6U6N) Rabbit mAb.

Background

Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). These processes are largely controlled by mitochondrial dynamin-related GTPases, including mitofusin-1, mitofusin-2, OPA1, and DRP1. DRP1 regulates mitochondrial fission, while the mitofusins and OPA1 control fusion at the outer and inner mitochondrial membrane, respectively.

OPA1, or Optic Atrophy 1, was originally identified as a genetic cause for Autosomal Dominant Optic Atrophy, a neuropathy resulting in progressive visual loss (2,3). OPA1 is a widely expressed protein localized to the inner mitochondrial membrane, which regulates mitochondrial fusion and cristae morphology and protects against apoptosis (4-6). OPA1 activity is tightly regulated through alternative splicing and post-translational modifications including complex proteolytic processing by multiple proteases (7-12). In addition, OPA1 expression can be induced under conditions of metabolic demand through a pathway involving Parkin induced NF-κB activation (13).

  1. Kasahara, A. and Scorrano, L. (2014) Trends Cell Biol 24, 761-70.
  2. Delettre, C. et al. (2000) Nat Genet 26, 207-10.
  3. Alexander, C. et al. (2000) Nat Genet 26, 211-5.
  4. Frezza, C. et al. (2006) Cell 126, 177-89.
  5. Olichon, A. et al. (2003) J Biol Chem 278, 7743-6.
  6. Griparic, L. et al. (2004) J Biol Chem 279, 18792-8.
  7. Delettre, C. et al. (2001) Hum Genet 109, 584-91.
  8. Olichon, A. et al. (2007) Cell Death Differ 14, 682-92.
  9. Ishihara, N. et al. (2006) EMBO J 25, 2966-77.
  10. Cipolat, S. et al. (2006) Cell 126, 163-75.
  11. Griparic, L. et al. (2007) J Cell Biol 178, 757-64.
  12. Merkwirth, C. et al. (2008) Genes Dev 22, 476-88.
  13. Müller-Rischart, A.K. et al. (2013) Mol Cell 49, 908-21.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications