Cell Signaling Technology

Product Pathways - Neuroscience

GRK3 (D8G6V) Rabbit mAb #80362

No. Size Price
80362S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
80362 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 80 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

GRK3 (D8G6V) Rabbit mAb recognizes endogenous levels of total GRK3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys454 of human GRK3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and various cell lines using GRK3 (D8G6V) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human GRK3 protein (hGRK3-Myc/DDK; +), using GRK3 (D8G6V) Rabbit mAb.


G-protein-coupled receptor kinase 3 (GRK3), also known as beta-adrenergic receptor kinase 2 (beta-ARK2), is a member of the GRK family, which phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR (1). GRK3 has been implicated in the phosphorylation of GPCRs, enabling their interaction with beta-arrestin, and facilitating their signaling through ERK1/2 phosphorylation (2). More recently, GRK3 was found to play a critical role in tumor progression through stimulation of angiogenesis; furthermore, GRK3 was found to be overexpressed in human prostate cancer, in particular in metastatic tumors (3).

  1. Pitcher, J.A. et al. (1998) Annu Rev Biochem 67, 653-92.
  2. Macey, T.A. et al. (2006) J Biol Chem 281, 34515-24.
  3. Li, W. et al. (2014) Proc Natl Acad Sci U S A 111, 1521-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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