Cell Signaling Technology

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Phospho-Catenin δ-1 (Ser320) Antibody #8016

No. Size Price
8016S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
8016 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 100 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey,

Specificity / Sensitivity

Phospho-Catenin δ-1 (Ser320) Antibody recognizes endogenous levels of catenin δ-1 protein only when phosphorylated at Ser320. Phospho-Catenin δ-1 (Ser320) Antibody检测仅在Ser320位点磷酸化的内源性catenin δ-1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser320 of human catenin δ-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. 通过人工合成人源catenin δ-1蛋白Ser320位点周围相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with λ phosphatase (+), using Phospho-Catenin δ-1 (Ser320) Antibody (upper) and Catenin δ-1 Antibody #4989 (lower). 使用Phospho-Catenin δ-1 (Ser320) Antibody (上图)和Catenin δ-1 Antibody #4989 (下图),免疫印迹(Western Blot)分析A-431细胞系Phospho-Catenin δ-1和Catenin δ-1蛋白水平,细胞分为未处理组(-)和λ phosphatase处理组(+)。

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). Catenin δ-1 (p120 catenin)蛋白有一个氨基末端卷曲螺旋结构域,接着是一个调节结构域,其含有许多磷酸化位点和一个十个连接的42氨基酸重复序列的中央 Armadillo重复区域。该蛋白羧基末端含有不知道的功能(1)。Catenin δ-1 蛋白在调节细胞与细胞的粘附实现关键角色,因为它调节E-cadherin蛋白在细胞表面周转从而确定可用于细胞与细胞粘附的E-cadherin蛋白水平(2) 。Catenin δ-1蛋白在钙粘蛋白介导的粘附有正性和负性的影响(3)。 Actin 动力学也通过catenin δ-1蛋白调节,其能RhoA、Rac和cdc42蛋白(1)。类似于β-catenin蛋白,catenin δ-1蛋白定位于细胞核,但是这个定位的功能仍不清楚。许多研究认为catenin δ-1蛋白在不同类型的肿瘤细胞中不相关被表达或缺乏,认为catenin δ-1蛋白可能作为肿瘤抑制因子的功能(4)。

Phosphorylation at Ser320 on catenin δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (5). Cell Signaling Technology (CST)公司使用PhosphoScan®, a CST™ LC-MS/MS平台发现磷酸化位点,catenin δ-1蛋白在Ser320位点磷酸化被鉴定 (5)。

  1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
  2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
  3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
  4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
  5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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