Product Pathways - PathScan ELISA
PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair #7945
H M R Hm Mk
|Product Includes||Volume||Solution Color|
|Alpha-Tubulin Capture Ab (100X)||0.4 ml|
|Alpha-Tubulin Detection Ab (100X)||0.4 ml|
|Anti-mouse IgG, HRP-linked Antibody||0.04 ml|
Specificity / Sensitivity
For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
对于抗体对的specificity和sensitivity，请参考相应的PathScan® Sandwich ELISA Kit。说明：因为通过内部实验确定，该试剂盒检测已知的物种，但是可能也会检测其它物种的同源性蛋白。
CST's PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total α-Tubulin Sandwich ELISA Kit #7944. Capture and detection antibodies (100X stocks) and an HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The α-tubulin rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an α-tubulin mouse detection antibody and HRP-linked, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of α-tubulin. Microtubule stabilizing or destabilizing agents may significantly increase or decrease the signal, respectively.
CST's PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair被提供作为PathScan® Total α-Tubulin Sandwich ELISA Kit #7944中一个十分经济产品。吸附和检测的抗体(100X stocks)以及HRP-conjugated secondary antibody (1000X stock)被提供。对于4 x 96 well ELISAs，充足的试剂被提供。α-tubulin rabbit在PBS中包被在微孔板上过夜。在封闭后，细胞裂解液加入之后是一个α-tubulin mouse detection antibody和HRP-linked, anti-mouse IgG antibody加入。HRP底物TMB被加入后显色。该显色的吸光度值的大小与α-tubulin蛋白量成比例。试剂盒里的抗体是针对该试剂盒特定制备的。
Figure 1. The relationship between the protein concentration of the lysate from A-431 cells and the absorbance at 450 nm using the PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair #7945 is shown.
图1： A-431细胞裂解的蛋白浓度与在450 nm吸光度的关系通过使用PathScan® Total α-Tubulin Sandwich ELISA Antibody Pair #7945被显示。
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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- 2125 α-Tubulin (11H10) Rabbit mAb
- 2144 α-Tubulin Antibody
- 2148 α/β-Tubulin Antibody
- 7002 STOP Solution
- 7004 TMB Substrate
- 7944 PathScan® Total α-Tubulin Sandwich ELISA Kit
- 9803 Cell Lysis Buffer (10X)
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
- 9998 BSA
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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