Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb #77699

CD31   PECAM-1   sc-101454   sc-1506  

No. Size Price
77699S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
77699T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
77699 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 135 Rabbit IgG
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb recognizes endogenous levels of total CD31 (PECAM-1) protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala451 of mouse CD31 (PECAM-1) protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A2058 xenograft using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 32D (CD31/PECAM-1 positive) and 3T3 (CD31/PECAM-1 negative) cells using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse liver using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse kidney using CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb.

Background

CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).

  1. Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
  2. Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-72.
  3. Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-24.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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